[通过组织化学酚氧化酶检测研究去污剂对人白细胞酚氧化酶活性的修饰作用]
[Modification of the phenoloxidase activity of human leukocytes by detergents, studied by histochemical phenoloxidase detection].
作者信息
Schmidt H, Anger A
机构信息
Institut für Biochemie, Martin-Luther-Universität, Halle, DDR.
出版信息
Acta Histochem. 1988;83(1):39-49.
PMID:3132017
Abstract
Using the histochemical detection for PO the in vitro effect of several detergents was tested. Dimethylsulfoxide, Triton X 100, Sodium desoxycholate, Tween 20, and Cetyltrimethyl ammonium bromide were investigated to check their effects upon the morphology and enzymatic activity of human leucocytes. Cetyltrimethyl ammonium bromide seems to be most suitable for the disintegration of cells. It does not inhibit the PO-activity.
摘要
通过对过氧化物酶（PO）进行组织化学检测，测试了几种去污剂的体外效果。研究了二甲基亚砜、吐温80、脱氧胆酸钠、吐温20和十六烷基三甲基溴化铵，以检查它们对人白细胞形态和酶活性的影响。十六烷基三甲基溴化铵似乎最适合细胞裂解，它不会抑制过氧化物酶活性。
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