Li Qiyan, Zhu Riran, Li Jun, Wang Xiaobing, Xu Lihua, Li Yanshen, Li Peng
Health Food and Cosmetics Laboratory, Shandong Institute for Food and Drug Control, Jinan 250101, China.
Faculty of Pharmaceutical Sciences, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250012, China.
Int J Anal Chem. 2019 Jun 20;2019:7131907. doi: 10.1155/2019/7131907. eCollection 2019.
A direct and highly specific chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) method for monitoring chloramphenicol (CAP) in cosmetics has been developed. The anti-chloramphenicol antibody (mAb) adopted in this work for direct immunoassay could bind to CAP specifically, with negligible cross-reactivity (CR) (less than 0.01%) with most CAP analogues, including structurally related thiamphenicol (TAP) and florfenicol (FF). The limit of detection (LOD), measured by IC, was 0.0021 ng mL. The detection range (IC-IC) was ranged from 0.00979 to 0.12026 ng mL. In spiked cosmetics samples, mean recoveries ranged from 82.7% to 99.6%, with intraday and interday variation less than 9.8 and 8.2%, respectively. Moreover, with the help of HRP-labeled anti-CAP mAb, the method could be processed in fast direct immunoreaction mode. This CL-ELISA method could be applied for specific, rapid, semiquantitative, and quantitative detection of CAP in cosmetics, facilitating the precise quality control of CAP contamination.
已开发出一种用于监测化妆品中氯霉素（CAP）的直接且高度特异性的化学发光酶联免疫吸附测定（CL-ELISA）方法。本研究采用的抗氯霉素抗体（单克隆抗体）用于直接免疫测定时，能特异性结合CAP，与大多数CAP类似物（包括结构相关的甲砜霉素（TAP）和氟苯尼考（FF））的交叉反应（CR）可忽略不计（小于0.01%）。通过免疫复合物（IC）测定的检测限（LOD）为0.0021 ng/mL。检测范围（IC20-IC80）为0.00979至0.12026 ng/mL。在加标化妆品样品中，平均回收率为82.7%至99.6%，日内和日间变化分别小于9.8%和8.2%。此外，借助辣根过氧化物酶（HRP）标记的抗CAP单克隆抗体，该方法可在快速直接免疫反应模式下进行。这种CL-ELISA方法可用于化妆品中CAP的特异性、快速、半定量和定量检测，有助于对CAP污染进行精确的质量控制。
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