Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Department of Bacteriology and Virology, Shiraz University of Medical Science, Shiraz, Iran.
Infect Disord Drug Targets. 2020;20(5):659-666. doi: 10.2174/1871526519666190715152118.
Antibiotic resistance, phylogenetic groups and Pulsed-Field Gel Electrophoresis (PFGE) patterns were evaluated in urinary tract infection (UTI) Escherichia coli (E. coli) isolates from outpatients and inpatients.
In this study, antibiotic resistance to E. coli isolated from non-hospitalized and hospitalized patients (153 outpatients and 147 inpatients ) was evaluated in Shiraz County, Iran. Phylogenetic groups and Pulse Field Gel Electrophoresis (PFGE) patterns of 143 ESBLs-producing E. coli were also assessed.
The prevalence of ESBL-producing E. coli was shown to be 46.4% and 49% in the outpatient and inpatient UTI E. coli isolates, respectively. Most ESBL-producers were detected on patients hospitalized in clinical surgery units (66.7%) and intensive care units (62.5%). Phylogenetic group D was the dominant group in both the outpatient and inpatient isolates (67.6% and 61.1%, respectively) and also in internal, clinical surgery and ICU units. PFGE results showed more relatedness (>80% similarity) among inpatient isolates. PFGE analysis of 49 ESBL-producing inpatient E.coli in hospital units revealed 17 different pulsotypes, consisting of 11 clones and 6 single patterns. There were no clonal patterns in outpatient isolates, and similarity among the outpatient isolates and also between inpatient and outpatient isolates was less than 80% (75% and 66%, respectively).
The results showed extreme genomic diversity among the ESBL-producing E. coli isolates in terms of the community and multiclonal dissemination of ESBL-producing E. coli isolated from hospital units.
评估门诊和住院患者尿路感染（UTI）大肠埃希菌（E. coli）分离株的抗生素耐药性、系统发育群和脉冲场凝胶电泳（PFGE）模式。
在这项研究中，评估了伊朗锡拉兹县非住院和住院患者（153 名门诊患者和 147 名住院患者）分离的 E. coli 对各种抗生素的耐药性。还评估了 143 株产 ESBLs 的 E. coli 的脉冲场凝胶电泳（PFGE）模式和系统发育群。
门诊和住院 UTI E. coli 分离株中产 ESBLs 的 E. coli 的流行率分别为 46.4%和 49%。大多数产 ESBLs 的分离株存在于临床外科病房（66.7%）和重症监护病房（62.5%）的住院患者中。在门诊和住院分离株中，优势群均为 D 群（分别为 67.6%和 61.1%），也存在于内科、临床外科和 ICU 病房。PFGE 结果显示住院患者分离株之间的相关性更高（>80%相似性）。对住院病房 49 株产 ESBLs 的 E. coli 的 PFGE 分析显示，有 17 种不同的脉冲型，包括 11 种克隆和 6 种单一型。门诊分离株中没有克隆型，门诊分离株之间以及住院和门诊分离株之间的相似性均小于 80%（分别为 75%和 66%）。
结果表明，从医院病房分离的产 ESBLs 的 E. coli 分离株在社区和多克隆传播方面具有极高的基因组多样性。
