Biomedical Research Institute , National Institute of Advanced Industrial Science and Technology (AIST) , 1-1-1 Higashi , Tsukuba , Ibaraki 305-8565 , Japan.
Graduate School of Engineering , Tokyo University of Agriculture and Technology , 2-24-16 Nakamachi , Koganei , Tokyo 184-8588 , Japan.
Anal Chem. 2019 Aug 20;91(16):10557-10563. doi: 10.1021/acs.analchem.9b01569. Epub 2019 Aug 1.
Intercellular adhesion strengths between two kinds of murine breast cancer cells with different malignancies were measured quantitatively using a metal cup-attached chip with atomic force microscopy (AFM). The cup-attached chip was used to approach a cell, pick it up, and then approach another cell, and the adhesion strengths were measured according to the contact time of the cells between 0 to 60 s. Separation work was used as a parameter for quantitative comparisons of the strengths. As a result, the work of a highly metastatic cancer cell (FP10SC2) was greater than a low metastatic cancer cell (4T1-LM) throughout all contact times examined. Adhesion was analyzed from a point of a view of binding kinetics of receptors on cells, and two possibilities were found: one was the number of cell adhesive receptors increased, and the other was the work to separate single molecular binding increased with increasing cancer cell malignancy. These results indicated quantitative measurements of intercellular adhesion strengths using AFM yielded information to understand the mechanism of the cancer progression from a new perspective.
采用原子力显微镜（AFM）的金属杯贴附芯片定量测量了两种恶性程度不同的鼠乳腺癌细胞之间的细胞间黏附强度。使用杯贴附芯片接近一个细胞，将其拾起，然后接近另一个细胞，并根据细胞之间 0 到 60 秒的接触时间测量黏附强度。分离功被用作定量比较强度的参数。结果，在所有检查的接触时间内，高转移性癌细胞（FP10SC2）的功大于低转移性癌细胞（4T1-LM）。从细胞表面受体结合动力学的角度分析黏附，发现有两种可能性：一种是细胞黏附受体的数量增加，另一种是随着癌细胞恶性程度的增加，分离单个分子结合的功也增加。这些结果表明，使用 AFM 对细胞间黏附强度进行定量测量，可以从新的角度获得理解癌症进展机制的信息。
