miR-939-3p 的过表达预示着肺癌预后不良，并促进其进展。

Overexpression of miR-939-3p predicts poor prognosis and promotes progression in lung cancer.

机构信息

Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, Shandong 250117, China.

Department of Oncology, Binzhou Medical University Hospital, Binzhou, Shandong 256603, China.

出版信息

Cancer Biomark. 2019;25(4):325-332. doi: 10.3233/CBM-190271.

DOI:10.3233/CBM-190271

PMID:31322549

Abstract

BACKGROUND

Lung cancer is the main cancer-related deaths worldwide. In this study, we explored the clinical prognostic significance and functional role of miR-939-3p in lung cancer.

METHODS

We analyzed the expression of miR-939-3p in lung cancer tissues and cells by qRT-PCR. The prognostic significance of miR-939-3p was investigated using the Kaplan-Meier survival and Cox regression analyses. The CCK-8 assay was used to determine the role of miR-939-3p in cell proliferation. Transwell assays were used to determine the effects of miR-939-3p on cell migration and invasion abilities.

RESULTS

The expression of miR-939-3p was upregulated in cancer tissues and cell lines compared with adjacent normal tissues and normal cells, respectively. The upregulated miR-939-3p was significantly associated with lymph node metastasis, TNM stage and poor prognosis of lung cancer patients. After the transfection of miR-939 mimic, overexpression of miR-939-3p promoted lung cancer cell proliferation, migration, and invasion.

CONCLUSION

These findings suggested that miR-939-3p acts as an oncogene and promotes cell proliferation, migration, and invasion in lung cancer. miR-939-3p may be a potential independent prognostic biomarker in lung cancer.

摘要

背景

肺癌是全球主要的癌症相关死亡原因。在这项研究中，我们探讨了 miR-939-3p 在肺癌中的临床预后意义和功能作用。

方法

通过 qRT-PCR 分析肺癌组织和细胞中 miR-939-3p 的表达。通过 Kaplan-Meier 生存和 Cox 回归分析研究 miR-939-3p 的预后意义。CCK-8 测定法用于确定 miR-939-3p 在细胞增殖中的作用。Transwell 测定法用于确定 miR-939-3p 对细胞迁移和侵袭能力的影响。