关节软骨最大蛋白聚糖单体的蛋白质核心。凝胶电泳图谱及色氨酸肽键裂解

The protein core of the largest proteoglycan monomer of articular cartilage. Gel electrophoretic pattern and tryptophanyl peptide bond cleavage.

作者信息

Stanescu V, Chaminade F, Muriel M P

机构信息

Unité de Recherches de Génétique Médicale (I.N.S.E.R.M. U.12), Hôpital des Enfants-Malades, Paris, France.

出版信息

Connect Tissue Res. 1987;16(4):377-84. doi: 10.3109/03008208709005622.

DOI:10.3109/03008208709005622

PMID:3132351

Abstract

The largest proteoglycan monomer of baboon (Papio papio) articular cartilage was isolated and the protein rich core was obtained after chondroitinase AC II and keratanase digestions. On SDS-PAGE the core yielded a single band with apparent Mr of 290,000. Tryptophanyl peptide bond cleavage of the core with N-chlorosuccinimide/urea gave 4 peptides with apparent Mr of 105,000, 66,000, 62,000 and 56,000

摘要

分离出狒狒（巴氏狒狒）关节软骨中最大的蛋白聚糖单体，并在软骨素酶AC II和角蛋白酶消化后获得富含蛋白质的核心。在SDS - PAGE上，该核心产生了一条表观分子量为290,000的单带。用N - 氯代琥珀酰亚胺/尿素对该核心进行色氨酸肽键切割，得到了4条表观分子量分别为105,000、66,000、62,000和56,000的肽段。