Key Laboratory of Development and Application of Rural Renewable Energy, Biogas Institute of Ministry of Agriculture, Chengdu 610041, PR China.
College of Pharmacy and Biological Engineering, Chengdu University, No. 2205, Cheng Luo Road, Chengdu 610106, PR China.
Bioresour Technol. 2019 Oct;289:121741. doi: 10.1016/j.biortech.2019.121741. Epub 2019 Jul 2.
In this study, the effect of microalgal strains on the formation of algal-bacterial biofloc was investigated in liquid digestate pretreated by a sequencing batch reactor (SBR), which loaded much aerobic bacteria from activated sludge. Six microalgal strains resulted in three cases: no-bioflocculation (Scenedesmus obliquus and Botryococcus braunii), optimal-bioflocculation with high flocculation activity and good growth (Chlorella sp. BWY-1, Haematococcus pluvialis and Dictyosphaerium ehnenbergianum) and over-bioflocculation with high flocculation activity and bad growth (Chlorella vulgaris). Chlorella sp. BWY-1 provided a better level of flocculation activity and growth. Polysaccharides and proteins were present in EPS of algal-bacterial biofloc, and their distribution was confirmed by staining with alcian blue and fluorescein isothiocyanate (FITC).
在本研究中，考察了在序批式反应器（SBR）预处理的液体消化液中，微藻菌株对藻-菌生物絮体形成的影响，该 SBR 从活性污泥中加载了大量好氧菌。六种微藻菌株导致了三种情况：无生物絮体形成（斜生栅藻和杜氏藻）、高絮凝活性和好生长的最佳生物絮体形成（小球藻 BWY-1、雨生红球藻和胶球藻）和高絮凝活性和不良生长的过生物絮体形成（普通小球藻）。小球藻 BWY-1 提供了更好的絮凝活性和生长水平。藻-菌生物絮体的 EPS 中存在多糖和蛋白质，并通过用茜素蓝和异硫氰酸荧光素（FITC）染色来确认其分布。
