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胎儿器官培养中胸腺细胞的分化：缺乏证据表明原胸腺细胞表达的白细胞介素2受体具有功能作用。

Differentiation of thymocytes in fetal organ culture: lack of evidence for the functional role of the interleukin 2 receptor expressed by prothymocytes.

作者信息

Plum J, De Smedt M

机构信息

Laboratorium voor Bacteriologie en Virologie, Universitair Ziekenhuis, Gent, Belgium.

出版信息

Eur J Immunol. 1988 May;18(5):795-9. doi: 10.1002/eji.1830180521.

DOI:10.1002/eji.1830180521
PMID:3132397
Abstract

Thymic rudiments of 14-day-old mouse embryos were put into organ culture in the presence of interleukin 2 (IL2) and PC.61, a monoclonal antibody that binds to the IL2 receptor (IL2R). After 5 and 7 days of culture, we found no influence of PC.61 on the growth of the thymus and the composition of the thymocyte subpopulations as studied with a panel of monoclonal antibodies. High-dose IL2 treatment of the organ culture resulted in a reduction of the number of thymocytes and a decrease in the single CD4+ and CD4+CD8+ thymocytes, whereas the number of CD8+Ly-1+ thymocytes and the number of CD4-CD8-Ly-1- IL2R+ thymocytes increased. The effect of high-dose IL2 treatment was ascribed to the induction of a nonspecific LAK activity. Our findings argue against a functional role of IL2R on prothymocytes during early T cell development.

摘要

将14日龄小鼠胚胎的胸腺原基置于白细胞介素2（IL2）和PC.61（一种与IL2受体（IL2R）结合的单克隆抗体）存在的器官培养体系中。培养5天和7天后，我们发现，使用一组单克隆抗体研究发现，PC.61对胸腺生长和胸腺细胞亚群组成没有影响。对器官培养体系进行高剂量IL2处理导致胸腺细胞数量减少，单个CD4+和CD4+CD8+胸腺细胞数量减少，而CD8+Ly-1+胸腺细胞数量和CD4-CD8-Ly-1-IL2R+胸腺细胞数量增加。高剂量IL2处理的效果归因于非特异性LAK活性的诱导。我们的研究结果表明，在早期T细胞发育过程中，IL2R对原胸腺细胞没有功能性作用。

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