白细胞介素-3和白细胞介素-4影响器官培养中的胸腺细胞分化。
IL-3 and IL-4 affect thymocyte differentiation in organ culture.
作者信息
Wood P M, Jordan R K, Givan A L, Brooks C G
机构信息
Division of Immunology, Medical School, Newcastle upon Tyne, U.K.
出版信息
Immunology. 1990 Sep;71(1):83-9.
The ability of lymphokines to affect the development and differentiation of mouse thymocytes in vitro was evaluated in a carefully controlled 3-day organ culture system. Concanavalin A (Con A)-induced supernatant (SN) from the T-cell clone D10.G4, which contains high concentrations of interleukin-3 (IL-3), IL-4 and IL-5, but lacks IL-1, IL-2 and interferon (IFN), markedly increased the proportion of CD4+CD8- cells, and decreased the proportion of CD4+CD8+ cells. These effects were unaffected by dialysing the SN, showing them to be caused by macromolecular factors. Highly purified recombinant IL-3 and IL-4 could exert similar effects, rIL-3 and rIL-4 both increasing the proportion of CD4+CD8- cells, and rIL4 in addition reducing the proportion of CD4+CD8+ cells. In conjunction with the findings of other investigators, these results indicate that at least four lymphokines (IL-1, IL-2, IL-3 and IL-4) can control T-cell development in the thymus.
在一个经过精心控制的3天器官培养系统中，评估了淋巴因子在体外影响小鼠胸腺细胞发育和分化的能力。来自T细胞克隆D10.G4的伴刀豆球蛋白A（Con A）诱导的上清液（SN），其含有高浓度的白细胞介素-3（IL-3）、IL-4和IL-5，但缺乏IL-1、IL-2和干扰素（IFN），显著增加了CD4+CD8-细胞的比例，并降低了CD4+CD8+细胞的比例。透析SN对这些效应没有影响，表明它们是由大分子因子引起的。高度纯化的重组IL-3和IL-4可发挥类似作用，rIL-3和rIL-4均增加了CD4+CD8-细胞的比例，此外rIL-4还降低了CD4+CD8+细胞的比例。结合其他研究者的发现，这些结果表明至少四种淋巴因子（IL-1、IL-2、IL-3和IL-4）可以控制胸腺中的T细胞发育。
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