Toyota Central R&D Lab, Nagakute, Aichi 480-1192, Japan.
J Biosci Bioeng. 2019 Dec;128(6):655-661. doi: 10.1016/j.jbiosc.2019.06.006. Epub 2019 Jul 16.
Metabolically engineered microorganisms that produce useful organic compounds will be helpful for realizing a sustainable society. The budding yeast Saccharomyces cerevisiae has high utility as a metabolic engineering platform because of its high fermentation ability, non-pathogenicity, and ease of handling. When producing yeast strains that produce exogenous compounds, it is a prerequisite to control the expression of exogenous enzyme-encoding genes. Terminator region in a gene expression cassette, as well as promoter region, could be used to improve metabolically engineered yeasts by increasing or decreasing the expression of the target enzyme-encoding genes. The findings on terminators have grown rapidly in the last decade, so an overview of these findings should provide a foothold for new developments.
代谢工程微生物生产有用的有机化合物将有助于实现可持续发展的社会。 budding 酵母酿酒酵母因其高发酵能力、非致病性和易于处理而具有很高的应用价值，是代谢工程平台。当生产产生外源化合物的酵母菌株时，控制外源酶编码基因的表达是前提条件。基因表达盒中的终止子区域以及启动子区域可用于通过增加或减少目标酶编码基因的表达来改善代谢工程酵母。过去十年中，关于终止子的研究进展迅速，因此对这些研究的概述应该为新的发展提供立足点。
