Handelsman D J, Boylan L M
Department of Medicine, University of Sydney, Australia.
J Clin Endocrinol Metab. 1988 Jul;67(1):175-9. doi: 10.1210/jcem-67-1-175.
The pulse frequency, amplitude, and mode of administration of GnRH all influence gonadotropin secretion and, ultimately, pituitary-gonadal function. We studied plasma LH responses to repetitive iv administration of GnRH given hourly for 5 h as a 2-microgram rapid (less than 15 s) bolus dose or a 2-microgram dose infused for 15 min of each hour in seven women deficient in endogenous GnRH and sex steroids. Plasma LH levels, measured at 10-min intervals throughout the 5-h period, rose more briskly (pattern X time course interactions: F = 3.33; P less than 0.0001) to higher levels overall (F = 11.7; P = 0.014) after rapid bolus GnRH administration than after GnRH infusion. Plasma FSH levels increased during both modes of delivery, with higher responses to rapid bolus GnRH administration (P = 0.005). Plasma estradiol levels did not change during either 5-h study. We conclude that the pattern of delivery of GnRH is a determinant of pituitary LH and FSH secretion in untreated hypogonadotropic women, and therefore, that alterations in the GnRH wave form and/or peak plasma GnRH concentrations consequent upon different rates of GnRH entry into the blood-stream may explain the different responses that occur when GnRH is given by different routes.
促性腺激素释放激素（GnRH）的脉冲频率、幅度及给药方式均会影响促性腺激素的分泌，最终影响垂体-性腺功能。我们研究了7名内源性GnRH和性类固醇缺乏的女性，她们每小时静脉注射GnRH共5小时，每次注射2微克，分别采用快速（小于15秒）推注剂量或每小时输注15分钟的2微克剂量，观察血浆促黄体生成素（LH）的反应。在整个5小时期间，每隔10分钟测量一次血浆LH水平，快速推注GnRH后，LH水平总体上升更快（模式X时间过程交互作用：F = 3.33；P < 0.0001），且升至更高水平（F = 11.7；P = 0.014），而GnRH输注后则不然。两种给药方式下血浆促卵泡生成素（FSH）水平均升高，快速推注GnRH给药后的反应更高（P = 0.005）。在5小时的研究期间，血浆雌二醇水平均未发生变化。我们得出结论，GnRH的给药方式是未治疗的低促性腺激素性女性垂体LH和FSH分泌的决定因素，因此，不同速率的GnRH进入血流后，GnRH波形和/或血浆GnRH峰值浓度的改变可能解释了不同给药途径给予GnRH时出现的不同反应。
