Department of Pharmaceutical Technology, Medical University of Gdansk, 107 Hallera Ave., 80-416, Gdansk, Poland.
Sci Rep. 2019 Jul 19;9(1):10525. doi: 10.1038/s41598-019-46987-y.
The purpose of the study was to determine the maximum safe concentration of calcium and phosphate in neonatal parenteral nutrition (PN) solutions when various combinations of inorganic and organic salts are applied. Twelve PN solutions for neonatal use were aseptically prepared. Increasing concentrations of inorganic and organic calcium and phosphate were added to the standard formulas. Each admixture was separately tested according to the following conditions; after mixing, at 37 °C for 24 hr, and the maximum safe combination of calcium and phosphate were stored at 4 °C for 30 days and followed by 24 hr at 37 °C. Visual inspections and microscopic observation of undiluted PN solutions as well as the membrane filter after filtration of the PN solution, pH evaluation, and absorbance were examined. The safe maximum concentration of organic and inorganic calcium and phosphate were proposed individually for each composition of parenteral nutrition solutions. Surprisingly, organic calcium with organic phosphate showed precipitation but over the therapeutic range. The protective effect of amino acid was observed and higher concentrations of calcium and phosphate were free of precipitation. This work is valuable in daily practice as it allows an increase in the limits of calcium and phosphate in PN solutions for infants.
这项研究的目的是确定在应用各种无机盐和有机盐组合时，新生儿肠外营养（PN）溶液中钙和磷的最大安全浓度。无菌制备了 12 种用于新生儿的 PN 溶液。将无机和有机钙和磷的浓度逐渐增加到标准配方中。根据以下条件分别测试每种混合物; 混合后，在 37°C 下放置 24 小时，然后将最大安全的钙和磷组合在 4°C 下储存 30 天，随后在 37°C 下放置 24 小时。检查未稀释的 PN 溶液的目视检查和显微镜观察以及 PN 溶液过滤后的膜过滤器、pH 值评估和吸光度。为每种肠外营养溶液的组成分别提出了有机和无机钙和磷的安全最大浓度。令人惊讶的是，有机钙与有机磷虽然在治疗范围内但出现沉淀。观察到氨基酸的保护作用，并且更高浓度的钙和磷没有沉淀。这项工作在日常实践中很有价值，因为它允许增加婴儿 PN 溶液中钙和磷的限制。
