Hu M L, Viljoen A J, Tappel A L
Department of Food Science and Technology, University of California, Davis 95616.
J Inorg Biochem. 1988 Apr;32(4):281-90. doi: 10.1016/0162-0134(88)85008-6.
Rats injected with aurothioglucose (ATG) for 5 days were subsequently injected with [75Se]selenious acid and killed after 3 days. Kidney and liver cytosols were chromatographed on Sephadex G-150. 75Se in kidney was associated with high molecular weight (HMW), 85,000 Mr, 26,000 Mr, and 10,000 Mr proteins and with a nonprotein fraction. The elution profile of liver cytosol was similar to that of kidney, but without a 26,000 Mr protein. ATG injection increased the association of 75Se with all fractions of kidney cytosol except the 85,000 Mr fractions, which contained Se-glutathione peroxidase (SeGSHPx) activity; 75Se in liver was increased only in HMW fractions. Unfractionated kidney cytosolic SeGSHPx activity was decreased 14% by ATG injection, but liver enzyme activity was not changed. However, Sephadex G-150 chromatography showed that total and specific activities, respectively, were decreased 28 and 23% in kidney and 25 and 16% in liver. Au coeluted with HMW and 10,000 Mr 73Se-containing kidney proteins; the latter contained 50% of the Au eluted from the column. DEAE Sephacel chromatography of the 10,000 Mr kidney protein showed that both Au and 75Se were tightly associated with metallothionein-like proteins. This study demonstrates the interaction of Au with rat liver and kidney 75Se-containing proteins.
给大鼠注射硫代葡萄糖金（ATG）5天后，接着注射[75Se]亚硒酸，并在3天后处死。将肾脏和肝脏的胞质溶胶在葡聚糖凝胶G - 150上进行层析。肾脏中的75Se与高分子量（HMW）、分子量85,000、26,000和10,000的蛋白质以及一个非蛋白质部分相关联。肝脏胞质溶胶的洗脱图谱与肾脏相似，但没有分子量为26,000的蛋白质。注射ATG增加了75Se与肾脏胞质溶胶所有部分的结合，但含有硒谷胱甘肽过氧化物酶（SeGSHPx）活性的85,000分子量部分除外；肝脏中的75Se仅在高分子量部分增加。未分级的肾脏胞质溶胶SeGSHPx活性因注射ATG而降低了14%，但肝脏酶活性没有变化。然而，葡聚糖凝胶G - 150层析显示，肾脏中总活性和比活性分别降低了28%和23%，肝脏中分别降低了25%和16%。金与高分子量和分子量为10,000且含有73Se的肾脏蛋白质共洗脱；后者含有从柱上洗脱的金的50%。对分子量为10,000的肾脏蛋白质进行二乙氨基乙基葡聚糖凝胶纤维素（DEAE Sephacel）层析表明，金和75Se都与类金属硫蛋白紧密结合。这项研究证明了金与大鼠肝脏和肾脏中含75Se的蛋白质之间的相互作用。
