Fish Genetics and Biotechnology Division, ICAR - Central Institute of Freshwater Aquaculture, Bhubaneswar, Odisha 751 002, India.
Centre for Agricultural Bioinformatics (CABin), ICAR-Indian Agricultural Statistics Research Institute, Library Avenue, PUSA, New Delhi 10012, India.
Genomics. 2020 Jan;112(1):32-44. doi: 10.1016/j.ygeno.2019.07.010. Epub 2019 Jul 17.
The role of microRNA in gene regulation during developmental biology has been well depicted in several organisms. The present study was performed to investigate miRNAs role in the liver tissues during carbohydrate metabolism and their targets in the farmed carp rohu, Labeo rohita, which is economically important species in aquaculture. Using Illumina-HiSeq technology, a total of 22,612,316; 44,316,046 and 13,338,434 clean reads were obtained from three small-RNA libraries. We have identified 138 conserved and 161 novel miRNAs and studies revealed that miR-22, miR-122, miR-365, miR-200, and miR-146 are involved in carbohydrate metabolism. Further analysis depicted mature miRNA and their predicted target sites in genes that were involved in developmental biology, cellular activities, transportation, etc. This is the first report of the presence of miRNAs in liver tissue of rohu and their comparative profile linked with metabolism serves as a vital resource as a biomarker.
在几个生物体中，miRNA 在发育生物学中的基因调控作用已经得到了很好的描述。本研究旨在研究 miRNA 在碳水化合物代谢过程中在罗非鱼肝组织中的作用及其在水产养殖中经济重要物种罗非鱼（Labeo rohita）中的靶标。使用 Illumina-HiSeq 技术，从三个小 RNA 文库中分别获得了 22612316、44316046 和 13338434 个清洁读数。我们鉴定了 138 个保守和 161 个新的 miRNA，研究表明 miR-22、miR-122、miR-365、miR-200 和 miR-146 参与碳水化合物代谢。进一步的分析描述了参与发育生物学、细胞活动、运输等的基因中的成熟 miRNA 及其预测靶位点。这是首次在罗非鱼肝组织中存在 miRNA 的报道，它们与代谢相关的比较图谱可作为生物标志物的重要资源。
