Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India; Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, 781035, Assam, India.
Environmental Biotechnology Laboratory, Life Sciences Division, Institute of Advanced Study in Science and Technology, Guwahati, 781035, Assam, India.
Colloids Surf B Biointerfaces. 2019 Oct 1;182:110358. doi: 10.1016/j.colsurfb.2019.110358. Epub 2019 Jul 10.
In this study, rhamnolipid (RL) production by Pseudomonas aeruginosa SS14 utilizing rice based Distillers Dried Grains with Solubles (rDDGS) as the sole carbon source was evaluated and the production parameters were optimized using response surface methodology. Highest RL (RL-rDDGS) yield was 14.87 g/L in a culture medium containing 12% (w/v) rDDGS and 11% (v/v) inoculum concentration after 48 h of fermentation at 35 °C. RL-rDDGS was produced as a mixture of mono and di-RL congeners with four novel homologues Rha-C, Rha-C, Rha-C, and Rha-Rha-C. The RL reduced the surface tension of water to 34.8 mN/m at a critical micelle concentration (CMC) value of 100 mg/L, exhibited high stability at a wide range of pH (6-12), heating time (0-120 min), and salinity (2-12% NaCl). Furthermore, RL-rDDGS demonstrated appreciable biofilm disruptive property against Candida tropicalis. This is the first report on the usage of rDDGS for sustainable and low cost production of RL.
在这项研究中，利用水稻为基础的干酒糟及其可溶物（rDDGS）作为唯一碳源，评估了铜绿假单胞菌 SS14 产生鼠李糖脂（RL）的情况，并利用响应面法优化了生产参数。在 35°C 发酵 48 小时后，在含有 12%（w/v）rDDGS 和 11%（v/v）接种浓度的培养基中，RL-rDDGS 的产量最高为 14.87g/L。RL-rDDGS 是由单和二 RL 同系物组成的混合物，有四个新的同系物 Rha-C、Rha-C、Rha-C 和 Rha-Rha-C。RL 在临界胶束浓度（CMC）值为 100mg/L 时将水的表面张力降低到 34.8mN/m，在宽 pH（6-12）、加热时间（0-120 分钟）和盐度（2-12%NaCl）范围内具有很高的稳定性。此外，RL-rDDGS 对热带假丝酵母表现出明显的生物膜破坏特性。这是首次报道使用 rDDGS 进行可持续和低成本生产 RL。
