Institute of Anatomy and Cell Biology I, Ludwig-Maximilian University (LMU), Munich, Germany; German Center for Vertigo and Balance Disorders, LMU, Munich, Germany; Graduate School of Systemic Neurosciences (GSN), LMU, Munich, Germany.
Institute of Anatomy and Cell Biology I, Ludwig-Maximilian University (LMU), Munich, Germany; German Center for Vertigo and Balance Disorders, LMU, Munich, Germany.
Prog Brain Res. 2019;249:117-123. doi: 10.1016/bs.pbr.2019.04.017. Epub 2019 Jun 7.
Potassium (K) channels are major contributors to fast and precise action potential generation. The aim of this study was to establish the immunoreactivity profile of several potassium channels in omnipause neurons (OPNs), which play a central role in premotor saccadic circuitry. To accomplish this, we histochemically examined monkey and human brainstem sections using antibodies against the voltage gated K-channels K1.1, K3.1b and K-Cl cotransporter (KCC2). We found that OPNs of both species were positive for all three K-antibodies and that the staining patterns were similar for both species. In individual OPNs, K3.1b was detected on the somatic membrane and proximal dendrites, while K1.1 was mainly confined to soma. Further, KCC2 immunoreactivity was strong in distal dendrites, but was weak in the somatic membrane. Our findings allow the speculation that the alterations in K-channel expression in OPNs could be the underlying mechanism for several saccadic disorders through neuronal and circuit-level malfunction.
钾通道是快速而精确的动作电位产生的主要贡献者。本研究的目的是确定在中央预动眼回路中起关键作用的全停顿神经元（OPN）中几种钾通道的免疫反应性特征。为了实现这一目标，我们使用针对电压门控钾通道 K1.1、K3.1b 和 K-Cl 共转运体（KCC2）的抗体对猴子和人类脑桥切片进行了组织化学检查。我们发现，这两个物种的 OPN 均对所有三种 K 抗体呈阳性，并且两种物种的染色模式相似。在单个 OPN 中，K3.1b 检测到在体细胞膜和近端树突上，而 K1.1 主要局限于体。此外，KCC2 免疫反应性在远端树突中较强，但在体细胞膜中较弱。我们的发现使人们推测，OPN 中钾通道表达的改变可能是通过神经元和电路水平的功能障碍导致几种扫视障碍的潜在机制。
