Komfo Anokye Teaching Hospital (KATH), Kumasi, Ghana.
Department of Medical Laboratory Technology, Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana.
Leuk Res. 2019 Sep;84:106189. doi: 10.1016/j.leukres.2019.106189. Epub 2019 Jul 12.
Platelet activation and functional changes in some haematological malignancies have been investigated with little or no known documentation on Burkitt lymphoma (BL). Abnormalities of platelets contribute to either haemorrhage or thrombotic episodes which are life-threatening in patients with BL. Thus, the study aimed at investigating the various platelet indices and platelet membrane glycoproteins in childhood Burkitt lymphoma. Platelet surface membrane glycoproteins (GPIIb/IIIa, P-selectin and GPIV using PAC 1, CD62p and CD36 monoclonal antibodies respectively) and platelet indices (Platelet Count [PLT], Plateletcrite [PCT], Mean Platelet Volume [MPV], Platelet Distribution Width [PDW] and Platelet Large Cell Ratio [P-LCR]) were determined in children with Burkitt lymphoma and healthy children (normal controls) based on flow cytometry and automated blood cell analysis techniques. PLT and PCT were higher in BL cases than in the normal controls with a significant difference in the PLT (P = 0.02). On the contrary, we observed a significant (p < 0.05) lower levels in the other platelet indices (MPV, PDW and P-LCR) in children with BL than the controls. With the exception of CD62 P, the other platelet membrane glycoproteins examined showed a decreased level of expression before and after the addition of an Adenosine -5- diphosphate (ADP) in cases of BL. In addition, PAC-1 was probably known to be associated with Burkitt Lymphoma (Odds Ratio [OR] 6.67, Relative Risk [RR] 3.13, 95% CI 1.06-9.21; p = 0.02). Finally, oral bleeding was observed to be the commonest bleeding episodes associated with childhood BL. Flow cytometry analysis and cell counting techniques of platelet assessment has described the expression of the platelet membrane glycoproteins and parameters in children with Burkitt lymphoma. Thus, children with Burkitt lymphoma tend to show normal to increased level of circulatory platelets but decreased platelet membrane glycoprotein expressions and platelet dysfunction.
血小板在一些血液恶性肿瘤中的活化和功能变化已经得到了研究，但关于伯基特淋巴瘤（BL）的研究却很少或没有。血小板的异常会导致出血或血栓形成，这对 BL 患者是危及生命的。因此，本研究旨在探讨儿童伯基特淋巴瘤中的各种血小板指标和血小板膜糖蛋白。使用 PAC-1、CD62p 和 CD36 单克隆抗体分别检测血小板表面膜糖蛋白（GPIIb/IIIa、P-选择素和 GPIV）和血小板指数（血小板计数[PLT]、血小板压积[PCT]、平均血小板体积[MPV]、血小板分布宽度[PDW]和血小板大细胞比[P-LCR]）在伯基特淋巴瘤患儿和健康儿童（正常对照组）中。与正常对照组相比，BL 患儿的 PLT 和 PCT 更高，PLT 有显著差异（P=0.02）。相反，我们观察到 BL 患儿的其他血小板指数（MPV、PDW 和 P-LCR）明显低于对照组（p<0.05）。除 CD62p 外，BL 患儿加入腺嘌呤二核苷酸（ADP）前后其他血小板膜糖蛋白的表达水平均降低。此外，PAC-1 可能与伯基特淋巴瘤有关（优势比[OR]6.67，相对风险[RR]3.13，95%可信区间[CI]1.06-9.21；p=0.02）。最后，观察到口腔出血是与儿童 BL 相关的最常见出血事件。血小板评估的流式细胞术分析和细胞计数技术描述了伯基特淋巴瘤患儿血小板膜糖蛋白和参数的表达。因此，伯基特淋巴瘤患儿的循环血小板水平通常正常或升高，但血小板膜糖蛋白表达和血小板功能下降。
