Köhler T, Landgraf J, Nuhn P
Sektion Pharmazie, Martin-Luther-Universität Halle-Wittenberg.
Pharmazie. 1988 Mar;43(3):178-84.
A coupled assay of phospholipase-A2 and lipoxygenase that especially can be applied to the determination of phospholipase-A2-inhibition is described. A partialsynthetic dilinolenoylglycerophosphocholine is used as substrate in the form of mixed micelles with Twenn-20. Pentadienoic fatty acids primarily produced by venom phospholipase-A2 are peroxydized in a second step quantitatively. Diminution in oxygen content is registrated by an oxygen sensitive electrode and the reaction process is plotted continuously. The usefulness of this assay in the screening of inhibitors and disturbing influences are discussed.
描述了一种特别适用于测定磷脂酶A2抑制作用的磷脂酶A2和脂氧合酶偶联测定法。部分合成的二亚麻酰甘油磷酸胆碱以与吐温-20形成混合胶束的形式用作底物。由毒液磷脂酶A2主要产生的戊二烯酸脂肪酸在第二步中被定量过氧化。氧含量的减少通过氧敏感电极记录，并且反应过程被连续绘制。讨论了该测定法在筛选抑制剂和干扰影响方面的实用性。
