Spices, and Flavour science Department.
Sensory Science Department, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570 020, India.
Nat Prod Res. 2021 Apr;35(7):1221-1225. doi: 10.1080/14786419.2019.1643863. Epub 2019 Jul 22.
Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography (GC-FID) was explored to determine the fingerprinting characteristic of L. volatiles to differentiate between different ginger species. The effect of different fibers [polydimethylsiloxane (PDMS, 7 μm), polyacrylate (PA, 85 μm)], different temperature and time on the HS-SPME-GC was investigated by using response surface methodology coupled with full factorial experimental design. The area percentage of the major sesquiterpenes (Zerumbone and α-humulene) were 56.3 ± 4.7% and 47.5 ± 27.2% with PA fiber, respectively at optimum condition of 70 °C and 30 min. Validation of the developed HS-SPME-GC method with limits of detection and quantification for zerumbone was 0.09 and 0.28 μg/g, respectively, demonstrating the suitable sensitivity of HS-SPME-GC method for the quantification of sesquiterpenes. Therefore, the simplicity of HS-SPME-GC makes it a convenient tool for qualitative and quantitative comparison of different ginger species targeting at the marker sesquiterpene molecule, zerumbone.
采用顶空固相微萃取（HS-SPME）结合气相色谱（GC-FID）技术，探索了不同纤维（聚二甲基硅氧烷（PDMS，7μm）、聚丙烯酸酯（PA，85μm））、不同温度和时间对 HS-SPME-GC 的影响，采用响应面法结合全因子实验设计。在最佳条件 70℃和 30min 下，PA 纤维对大倍半萜（莪术酮和α-葎草烯）的面积百分比分别为 56.3±4.7%和 47.5±27.2%。建立的 HS-SPME-GC 方法具有较低的检测限和定量限，莪术酮的检测限和定量限分别为 0.09 和 0.28μg/g，表明 HS-SPME-GC 方法对倍半萜的定量具有较高的灵敏度。因此，HS-SPME-GC 方法的简单性使其成为一种方便的定性和定量比较不同姜属植物的工具，针对特征倍半萜分子莪术酮。
