Siddique Halima, Al-Ghafari Ayat, Choudhry Hani, AlTurki Suzan, Alshaibi Huda, Al Doghaither Huda, Alsufiani Hadeil
1Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
2Experimental Biochemistry Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Genet Test Mol Biomarkers. 2019 Aug;23(8):509-514. doi: 10.1089/gtmb.2018.0308. Epub 2019 Jul 18.
Long noncoding RNAs (lncRNAs) have recently been recognized as a new layer of biological regulation. They participate in mRNA regulation and may be useful as prognostic factors and drug targets. Colorectal cancer (CRC) is a common tumor that is characterized by its high mortality rate. Despite improvements in screening of CRC, the prognosis is still poor. Therefore, there is an urgent need to develop effective biomarkers for the detection of CRC. This study was designed to measure the expression of several oncogenic lncRNAs, including PANDAR, MALAT1, PCAT6, CCAT1, UCA1, MEG3, CCAT2, and BCAR4, in blood samples of healthy individuals and CRC patients. Total RNA was isolated from whole blood of 63 CRC patients and 40 controls and the expression of the lncRNAs was determined by real-time polymerase chain reaction and measured by REST2009 software. All -values <0.05 were considered statistically significant. The results showed that the expression levels of MALAT1, CCAT1, and PANDAR were significantly upregulated with 1.86, 4.54, and 4.68-fold higher levels ( < 0.05), respectively, in the blood of CRC patients compared to the controls. However, the other lncRNAs examined were not significantly expressed differentially in CRC blood samples. The findings of this study suggest that the expression of MALAT1, CCAT1, and PANDAR in blood could serve as potential biomarkers for CRC prognosis.
长链非编码RNA（lncRNAs）最近被认为是生物调节的一个新层面。它们参与mRNA调节，可能作为预后因素和药物靶点。结直肠癌（CRC）是一种常见肿瘤，其特征是死亡率高。尽管CRC筛查有所改进，但预后仍然很差。因此，迫切需要开发有效的生物标志物用于CRC检测。本研究旨在测量健康个体和CRC患者血液样本中几种致癌lncRNAs的表达，包括PANDAR、MALAT1、PCAT6、CCAT1、UCA1、MEG3、CCAT2和BCAR4。从63例CRC患者和40例对照的全血中分离总RNA，通过实时聚合酶链反应测定lncRNAs的表达，并由REST2009软件进行测量。所有P值<0.05被认为具有统计学意义。结果显示，与对照组相比，CRC患者血液中MALAT1、CCAT1和PANDAR的表达水平显著上调，分别高出1.86倍、4.54倍和4.68倍（P<0.05）。然而，所检测的其他lncRNAs在CRC血液样本中表达差异不显著。本研究结果表明，血液中MALAT1、CCAT1和PANDAR的表达可作为CRC预后的潜在生物标志物。
