Henderson W R, Harley J B, Fauci A S, Chi E Y
Department of Medicine, University of Washington School of Medicine, Seattle 98195.
Br J Haematol. 1988 May;69(1):13-21. doi: 10.1111/j.1365-2141.1988.tb07596.x.
Eosinophil degranulation induced by the calcium ionophore A23187 and opsonized zymosan particles was examined ultrastructurally in peripheral blood cells obtained from patients with the hypereosinophilic syndrome. Unstimulated hypereosinophilic syndrome eosinophils contained altered, vacuolated cytoplasmic granules and large cytoplasmic crystalloid structures not seen in normal cells. By morphometric analysis of transmission electron micrographs, the hypereosinophilic syndrome eosinophils contained a larger percentage of smaller sized cytoplasmic granules than normal cells. The hypereosinophilic syndrome eosinophils, however, were capable of undergoing noncytotoxic degranulation after stimulation with either A23187 or opsonized zymosan. Hypereosinophilic syndrome eosinophil degranulation was characterized by fusion of the perigranular membranes of adjacent cytoplasmic granules and vesiculation of the fused granules. Granule contents were released intracellularly into vacuoles after ionophore stimulation and into phagosomes containing the ingested zymosan particles. Noncytotoxic extracellular release of eosinophil peroxidase (EPO) was also observed after cell stimulation by either A23187 or zymosan. The capacity of hypereosinophilic syndrome eosinophils to degranulate after appropriate stimulation with release of toxic granule constituents such as EPO and other basic proteins may be important in the tissue injury observed in this syndrome.
在从嗜酸性粒细胞增多综合征患者获取的外周血细胞中，利用钙离子载体A23187和调理酵母聚糖颗粒诱导嗜酸性粒细胞脱颗粒，并进行了超微结构检查。未受刺激的嗜酸性粒细胞增多综合征嗜酸性粒细胞含有改变的、空泡化的细胞质颗粒以及正常细胞中未见的大的细胞质晶体结构。通过对透射电子显微镜照片进行形态计量分析，嗜酸性粒细胞增多综合征嗜酸性粒细胞中较小尺寸的细胞质颗粒所占百分比高于正常细胞。然而，嗜酸性粒细胞增多综合征嗜酸性粒细胞在用A23187或调理酵母聚糖刺激后能够进行非细胞毒性脱颗粒。嗜酸性粒细胞增多综合征嗜酸性粒细胞脱颗粒的特征是相邻细胞质颗粒的颗粒周膜融合以及融合颗粒的小泡化。离子载体刺激后，颗粒内容物在细胞内释放到液泡中，而在含有摄入的酵母聚糖颗粒的吞噬体中释放。在用A23187或酵母聚糖刺激细胞后，还观察到嗜酸性粒细胞过氧化物酶（EPO）的非细胞毒性细胞外释放。嗜酸性粒细胞增多综合征嗜酸性粒细胞在适当刺激后脱颗粒并释放诸如EPO和其他碱性蛋白等有毒颗粒成分的能力，可能在该综合征中观察到的组织损伤中起重要作用。
