[MHC restriction of the anti-allotypic response of T-lymphocytes in vitro].

In vitro MHC restriction of antigen-specific T-cell proliferation to Igk-Ib allotype of Igk chain has been studied in inbred August rats, using polyclonal and monoclonal antibodies to RT-1 molecules. Igk-1b-specific proliferation of immune T cells was completely abrogated by alloantiserum specific for RT-1c (August) molecules. Monoclonal antibodies (MAb) to RT-1B ("1-A-like") molecules inhibited markedly the response (about 70-80% inhibition), while anti-RT-ID ("I-E-like") MAb caused but weak inhibition (about 25%). Thus, the data obtained demonstrate RT-1Bc molecule as a main restriction element of Igk-1b-specific T-cell response.