I2BC, Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay , 91198 , Gif-sur-Yvette cedex , France.
Ipsen , 28100 Dreux , France.
Langmuir. 2019 Aug 13;35(32):10648-10657. doi: 10.1021/acs.langmuir.9b01542. Epub 2019 Aug 2.
An important aspect of cells is their shape flexibility that gives them motion but also a high adaptation versatility to their environment. This shape versatility is mediated by different types of protein-membrane interactions among which electrostatic plays an important role. In the present work we examined the interaction between a small dicationic peptide, that possesses self-assembly properties, and lipid model membranes. The peptide, lanreotide, spontaneously forms nanotubes in water that have a strictly uniform diameter. In the current work, we show that the interaction between the cationic peptide and negatively charged bilayers of lipids induces the formation of myelin sheath-like structures that we call nanoscrolls. By deciphering the different steps of formation and the molecular structure of the self-assembly, we show how electrostatics modify the spontaneous peptide and lipid way of packing.
细胞的一个重要特征是其形状的灵活性，这使它们能够运动，并且具有很高的环境适应多功能性。这种形状的多功能性是由不同类型的蛋白质-膜相互作用介导的，其中静电起着重要作用。在本工作中，我们研究了一种具有自组装特性的小分子二价阳离子肽与脂质模型膜之间的相互作用。该肽，兰瑞肽，在水中自发形成具有严格均匀直径的纳米管。在目前的工作中，我们表明，阳离子肽与带负电荷的脂质双层之间的相互作用诱导形成类似于髓鞘的结构，我们称之为纳米卷。通过解析自组装的形成的不同步骤和分子结构，我们展示了静电如何改变肽和脂质的自发包装方式。
