School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141004, India.
Faculty Centre on Integrated Rural Development and Management, Ramakrishna Mission Vivekanada Educational and Research Institute, Narendrapur, Kolkata, 700103, India.
Genome. 2019 Nov;62(11):705-714. doi: 10.1139/gen-2019-0062. Epub 2019 Jul 22.
Kotschy ex Steud. (BB, 2 = 24) is a wild species of rice that has many useful agronomic traits. An interspecific hybrid (AB, 2 = 24) was produced by crossing and variety Punjab Rice 122 (PR122, AA, 2 = 24) to broaden the narrow genetic base of cultivated rice. Cytological analysis of the pollen mother cells (PMCs) of the interspecific hybrids confirmed that they have 24 chromosomes. The F hybrids showed the presence of 19-20 univalents and 1-3 bivalents. The interspecific hybrid was treated with colchicine to produce a synthetic amphiploid (AABB, 2 = 48). Pollen fertility of the synthetic amphiploid was found to be greater than 50% and partial seed set was observed. Chromosome numbers in the PMCs of the synthetic amphiploid were 24II, showing normal pairing. Flow cytometric analysis also confirmed doubled genomic content in the synthetic amphiploid. Leaf morphological and anatomical studies of the synthetic amphiploid showed higher chlorophyll content and enlarged bundle sheath cells as compared with both of its parents. The synthetic amphiploid was backcrossed with PR122 to develop a series of addition and substitution lines for the transfer of useful genes from with least linkage drag.
Kotschy ex Steud.（BB，2 = 24）是一种野生稻种，具有许多有用的农艺性状。通过将 和 Punjab Rice 122 品种（PR122，AA，2 = 24）杂交，产生了一个种间杂种（AB，2 = 24），以拓宽栽培稻的狭窄遗传基础。对种间杂种花粉母细胞（PMCs）的细胞学分析证实，它们具有 24 条染色体。F1 杂种表现出 19-20 条单价体和 1-3 条二价体的存在。对种间杂种进行秋水仙碱处理，产生了一个合成的双二倍体（AABB，2 = 48）。合成的双二倍体的花粉育性发现大于 50%，并观察到部分结实。合成的双二倍体 PMCs 的染色体数为 24II，表现出正常的配对。流式细胞术分析也证实了合成的双二倍体基因组含量加倍。与双亲相比，合成的双二倍体的叶片形态和解剖学研究表明，叶绿素含量更高，束鞘细胞增大。合成的双二倍体与 PR122 回交，以开发一系列的添加和替代系，用于从 Kotschy ex Steud.（BB，2 = 24）中转移有用基因，而不会有最小的连锁累赘。
