Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil.
Laboratory of Plant Biochemistry, Department of Biochemistry, University of Maringá, 87020-900, PR, Brazil.
Plant Physiol Biochem. 2019 Sep;142:275-282. doi: 10.1016/j.plaphy.2019.07.015. Epub 2019 Jul 12.
Biomimetically incorporated into the lignin structure, rosmarinic acid improves in vitro maize cell wall saccharification; however, no in planta studies have been performed. We hypothesized that rosmarinic acid, itself, could inducer saccharification without disturbing plant growth. Its effects on growth, enzymes of the phenylpropanoid pathway, lignin, monomeric composition, and saccharification of maize were evaluated. In a short-term (24 h) exposure, rosmarinic acid caused deleterious effects on maize roots, inhibiting the first enzymes of the phenylpropanoid pathway, phenylalanine ammonia-lyase and tyrosine ammonia-lyase, altering lignin composition and slightly increasing saccharification. In a long-term (14 d) exposure, rosmarinic acid increased saccharification of maize stems by about 50% without any deleterious effects on plant growth, the phenylpropanoid pathway and lignin formation. This demonstrated that exogenous application of rosmarinic acid on maize plants improved saccharification, and represented an interesting approach in facilitating enzymatic hydrolysis of biomass polysaccharides and increasing bioethanol production.
迷迭香酸被生物整合到木质素结构中，可提高体外玉米细胞壁的糖化率；然而，尚未进行体内研究。我们假设迷迭香酸本身可以在不干扰植物生长的情况下诱导糖化。评估了迷迭香酸对玉米生长、苯丙烷途径酶、木质素、单体组成和糖化的影响。在短期（24 小时）暴露中，迷迭香酸对玉米根造成有害影响，抑制苯丙烷途径的第一酶，苯丙氨酸解氨酶和酪氨酸解氨酶，改变木质素组成并略微增加糖化。在长期（14 天）暴露中，迷迭香酸使玉米茎的糖化率提高了约 50%，而对植物生长、苯丙烷途径和木质素形成没有任何有害影响。这表明，外源施用迷迭香酸可提高玉米糖化率，为促进生物质多糖的酶解和提高生物乙醇产量提供了一种有趣的方法。
