Gawlik-Dziki Urszula, Luty Marcin, Dziki Dariusz, Świeca Michał, Piwowarczyk Katarzyna, Złotek Urszula, Czyż Jarosław
Department of Biochemistry and Food Chemistry, University of Life Sciences, Skromna Str. 8, 20-704 Lublin, Poland.
Department of Cell Biology, Faculty of Biophysics, Biochemistry and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-378 Kraków, Poland.
Antioxidants (Basel). 2019 Jul 19;8(7):228. doi: 10.3390/antiox8070228.
The proliferation and motile activity of prostate epithelial (Pnt2) and cancer cells (DU-145; PC-3) in the presence of bioavailable compounds from green coffee beans (GCB), wholemeal wheat bread (WMWB), and its GCB-fortified variant were analyzed. The considerable cytostatic and anti-invasive activity of GCB extracts was correlated with its phenolic contents. WMWB extract contained significantly lower levels of phenolics but still displayed relatively high cytostatic activity. However, the cytostatic properties of WMWB compounds were hardly augmented by 3% GCB flour supplementation. The cytoprotective activity of the WMWB compounds exerts a negative impact on the cytostatic activity of GCB compounds. These data confirm the relatively high chemopreventive potential of GCB. However, they also indicate that subtle interactions between bioavailable compounds in GCB and WMWB can negatively affect the nutraceutic potential of the fortified bread. Apparently, gastrointestinal processing differentially regulates the availability of individual compounds and affects the balance between the cytostatic and cytoprotective activity of the whole product. Our data show that comprehensive research is necessary before the fortification of a specific carrier with a specific supplement can be recommended.
分析了前列腺上皮细胞（Pnt2）和癌细胞（DU - 145；PC - 3）在存在来自绿咖啡豆（GCB）、全麦面包（WMWB）及其GCB强化变体的生物可利用化合物时的增殖和运动活性。GCB提取物显著的细胞抑制和抗侵袭活性与其酚类成分相关。WMWB提取物中的酚类含量明显较低，但仍表现出相对较高的细胞抑制活性。然而，添加3%的GCB面粉几乎没有增强WMWB化合物的细胞抑制特性。WMWB化合物的细胞保护活性对GCB化合物的细胞抑制活性产生负面影响。这些数据证实了GCB具有较高的化学预防潜力。然而，它们也表明GCB和WMWB中生物可利用化合物之间的微妙相互作用可能会对强化面包的营养保健潜力产生负面影响。显然，胃肠道加工会不同程度地调节单个化合物的可利用性，并影响整个产品细胞抑制和细胞保护活性之间的平衡。我们的数据表明，在推荐用特定补充剂强化特定载体之前，有必要进行全面的研究。
