转录因子 HBP1：调节前体脂肪细胞衰老和凋亡的因子。
Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes.
机构信息
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, 150030, Heilongjiang, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, 150030, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, 150030, Heilongjiang, China; College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
出版信息
Biochem Biophys Res Commun. 2019 Sep 17;517(2):216-220. doi: 10.1016/j.bbrc.2019.07.048. Epub 2019 Jul 20.
BACKGROUND
/aim: HMG-box protein 1 (HBP1) plays an important role in the senescence and apoptosis of mammalian cells, but its role in chicken cells remains unclear. The aim of this study was to investigate the effects of HBP1 on senescence and apoptosis of chicken preadipocytes.
METHODS
The immortalized chicken preadipocyte cell line (ICP2) was used as a cell model. Chicken HBP1 knockout and overexpressing preadipocyte cell lines were established using CRISPR/Cas9 gene editing technology and lentiviral infection. Western blotting was used to detect the protein expression of HBP1 and senescence markers p16 and p53. Cell senescence was measured by Sa-β-Gal staining and apoptosis was detected by flow cytometry.
RESULTS
HBP1 was highly expressed in senescent ICP2 cells compared with young ICP2 cells. After the deletion of HBP1, the degree of senescence, the apoptosis rate and the protein expression levels of p16 and p53 were significantly reduced. After the overexpression of HBP1, the degree of senescence, the apoptosis rate and the protein expression levels of p16 and p53 were significantly increased.
CONCLUSION
HBP1 promotes the senescence and apoptosis of chicken preadipocytes.
背景
HMG-盒蛋白 1（HBP1）在哺乳动物细胞的衰老和凋亡中发挥重要作用，但它在鸡细胞中的作用尚不清楚。本研究旨在探讨 HBP1 对鸡前体脂肪细胞衰老和凋亡的影响。
方法
使用永生化鸡前体脂肪细胞系（ICP2）作为细胞模型。利用 CRISPR/Cas9 基因编辑技术和慢病毒感染，建立了鸡 HBP1 敲除和过表达前体脂肪细胞系。通过 Western blot 检测 HBP1 和衰老标志物 p16 和 p53 的蛋白表达。通过 Sa-β-Gal 染色检测细胞衰老，通过流式细胞术检测细胞凋亡。
结果
与年轻的 ICP2 细胞相比，衰老的 ICP2 细胞中 HBP1 表达水平较高。敲除 HBP1 后，衰老程度、凋亡率以及 p16 和 p53 的蛋白表达水平均显著降低。过表达 HBP1 后，衰老程度、凋亡率以及 p16 和 p53 的蛋白表达水平均显著升高。
结论
HBP1 促进鸡前体脂肪细胞的衰老和凋亡。
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