Aix Marseille University, IRD, APHM, SSA, VITROME, Marseille, France.
Institut Hospitalo-Universitaire Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.
Arch Microbiol. 2019 Dec;201(10):1361-1368. doi: 10.1007/s00203-019-01704-1. Epub 2019 Jul 22.
Strain 6021061333 was isolated from the sputum of 16-year-old girl with cystic fibrosis following a pulmonary exacerbation. This bacterial strain could not be identified by our systematic MALDI-TOF mass spectrometry screening on a MicroFlex. This led to the sequencing of the 16S rRNA gene, which shows 97.83% sequence identity with Chryseobacterium kwangjuense strain KJ1R5, the phylogenetic closely related type strain of a species with standing in nomenclature, which putatively classifies it as a new species. Colonies are yellow, circular and 0.5-1 mm in diameter after cultivation at 28 °C for 24 h on 5% sheep blood-enriched Colombia agar. Growth occurs at temperatures in the range of 28-37 °C (optimally at 28 °C). Strain 6021061333 is Gram-negative, non-motile and strictly aerobic bacillus. It is catalase and oxidase positive. The 4,864,678 bp-long genome, composed of five contigs, has a G+C content of 38.86%. Out of the 4427 predicted genes, 4342 were protein-coding genes and 85 were RNAs. The major fatty acids are branched (13-methyl-tetradecanoic acid and 15-methyl-hexadecenoic acid). Digital DNA-DNA hybridization (dDDH) estimation and average nucleotide identity (ANI) of the strain 6021061333 against genomes of the type strains of related species ranged between 23.60 and 50.40% and between 79.31 and 93.06%, respectively. According to our taxonogenomics results, we propose the creation of Chryseobacterium phocaeense sp. nov. that contains the type strain 6021061333 (= CSUR P2660, = CECT 9670).
6021061333 菌株是从一名患有囊性纤维化的 16 岁女孩的痰中分离出来的，当时她处于肺部恶化期。我们通过 MicroFlex 上的系统 MALDI-TOF 质谱筛选无法识别该细菌菌株，这导致了 16S rRNA 基因的测序，该基因与光金黄杆菌 KJ1R5 菌株显示出 97.83%的序列同一性，后者是具有命名地位的密切相关的模式种的亲缘关系类型菌株，这使其被假定为一个新物种。在 28°C 下培养 24 小时后，在 5%羊血富集哥伦比亚琼脂上，菌落呈黄色，圆形，直径为 0.5-1mm。生长温度范围为 28-37°C（最佳温度为 28°C）。6021061333 菌株是革兰氏阴性、非运动、严格需氧杆菌。它是过氧化氢酶和氧化酶阳性的。基因组长 4864678bp，由五个连续序列组成，GC 含量为 38.86%。在 4427 个预测基因中，有 4342 个是编码蛋白的基因，85 个是 RNA。主要脂肪酸为支链脂肪酸（13-甲基十四烷酸和 15-甲基十六烯酸）。该菌株与相关种模式菌株的基因组之间的数字 DNA-DNA 杂交（dDDH）估计值和平均核苷酸同一性（ANI）分别在 23.60%至 50.40%和 79.31%至 93.06%之间。根据我们的分类基因组学结果，我们建议创建光金黄杆菌属 phocaeense sp. nov.，其中包含模式菌株 6021061333（=CSUR P2660，=CECT 9670）。
