Yano M, Hashimoto F, Sato M, Ohboshi C, Hirota M, Oshima I, Ohe Y, Shin S, Shima K
Department of Laboratory Medicine, Tokushima University School of Medicine, Japan.
Diabetologia. 1988 Apr;31(4):221-4. doi: 10.1007/BF00290588.
A radioimmunoassay using antibody against glucitol-lysine was developed to quantitate glycated proteins in the lens of diabetic rats. The amount of glycated protein was expressed as molar equivalents of reduced glycated hippuryl lysine (GlcRED-Hip-Lysine). Significant differences (p less than 0.01) were found in the amounts of glycated protein in the lenses of rats with streptozotocin-induced diabetes (3.92 +/- 0.59 nmol/mg protein, n = 5), those with streptozotocin-induced diabetes treated with insulin (2.94 +/- 0.36 nmol/mg protein, n = 4) and normal rats (1.23 +/- 0.22 nmol/mg protein, n = 5). There was a significant correlation between the concentration of glycated protein in the lens and the HbA1c level at the end of the 12 week experiment (r = 0.957, p less than 0.001). These results indicate that glycation of lens protein is parallel with the severity of diabetes in rats.
开发了一种使用抗葡糖醇赖氨酸抗体的放射免疫分析法，用于定量糖尿病大鼠晶状体中的糖化蛋白。糖化蛋白的量以还原糖化马尿酸赖氨酸（GlcRED-Hip-Lysine）的摩尔当量表示。在链脲佐菌素诱导的糖尿病大鼠（3.92±0.59 nmol/mg蛋白质，n = 5）、接受胰岛素治疗的链脲佐菌素诱导的糖尿病大鼠（2.94±0.36 nmol/mg蛋白质，n = 4）和正常大鼠（1.23±0.22 nmol/mg蛋白质，n = 5）的晶状体中，糖化蛋白的量存在显著差异（p小于0.01）。在12周实验结束时，晶状体中糖化蛋白的浓度与HbA1c水平之间存在显著相关性（r = 0.957，p小于0.001）。这些结果表明，大鼠晶状体蛋白的糖基化与糖尿病的严重程度平行。
