福赛拟杆菌刺激下人口腔角质细胞中白细胞介素-24 的调控。
Regulation of IL-24 in human oral keratinocytes stimulated with Tannerella forsythia.
机构信息
Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Korea.
Institute of Pharmaceutical Sciences, College of Pharmacy, Gachon University, Incheon, Korea.
出版信息
Mol Oral Microbiol. 2019 Oct;34(5):209-218. doi: 10.1111/omi.12265. Epub 2019 Aug 19.
Interleukin-24 is a pleiotropic immunoregulatory cytokine and a member of the IL-20R subfamily of the IL-10 family. The aim of this study was to investigate the regulation of IL-24 in the human oral keratinocyte cell line HOK-16B following infection with Tannerella forsythia, a major periodontal pathogen. T. forsythia induced the expression of IL-24 mRNA and the secretion of glycosylated IL-24 in HOK-16B cells. Glycosylation of IL-24 is linked to its solubility and bioavailability. T. forsythia-stimulated reactive oxygen species (ROS) induced the expression of IL-24, which was regulated by IL-6. The ROS inhibitor N-acetylcysteine and MAPK inhibitors significantly reduced the expression of IL-6 and IL-24 induced by T. forsythia. Recombinant human IL-24 significantly enhanced the expression of IL-1α, IL-8, CXCL10, and MCP-1 in HOK-16B cells. Together, these results indicate that ROS, MAPKs, and IL-6 comprise the axis of IL-24 expression in HOK-16B cells stimulated with T. forsythia. Thus, IL-24 may be involved in inflammation in oral keratinocytes.
白细胞介素-24 是一种多效免疫调节细胞因子，属于白细胞介素-10 家族的 IL-20R 亚家族。本研究旨在探讨人口腔角质细胞系 HOK-16B 在感染主要牙周病原体福赛斯坦纳菌（Tannerella forsythia）后 IL-24 的调节作用。T. forsythia 诱导 HOK-16B 细胞中 IL-24 mRNA 的表达和糖基化 IL-24 的分泌。IL-24 的糖基化与其可溶性和生物利用度有关。T. forsythia 刺激的活性氧（ROS）诱导了 IL-24 的表达，其受到 IL-6 的调节。ROS 抑制剂 N-乙酰半胱氨酸和 MAPK 抑制剂显著降低了 T. forsythia 诱导的 IL-6 和 IL-24 的表达。重组人 IL-24 显著增强了 HOK-16B 细胞中 IL-1α、IL-8、CXCL10 和 MCP-1 的表达。综上所述，这些结果表明，ROS、MAPKs 和 IL-6 构成了 T. forsythia 刺激 HOK-16B 细胞中 IL-24 表达的轴。因此，IL-24 可能参与口腔角质细胞的炎症反应。
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