Janecki A, Jakubowiak A, Steinberger A
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Texas Medical School, Houston 77030.
J Androl. 1988 Mar-Apr;9(2):126-32. doi: 10.1002/j.1939-4640.1988.tb01024.x.
The influence of germ cells (greater than 85% pachytene spermatocytes) on vectorial secretion of androgen binding protein (ABP) and transferrin by immature rat Sertoli cells was investigated using two-compartment culture chambers. The ratio of ABP secreted into the outer and inner compartment in control cultures of Sertoli cells alone was 1.9, and was not influenced by either FSH or testosterone. Co-culture of Sertoli cells in direct contact with germ cells in the presence of FSH decreased this ratio, the decrease being most pronounced (0.7) after 2 days of co-culture. This effect was not observed if the germ cells were not in direct contact with Sertoli cell monolayers. The outer to inner compartment ratio of transferrin in Sertoli cell-alone cultures was 1.6 and, in contrast to ABP, was not significantly influenced by the addition of germ cells, even in the presence of FSH. It is concluded that in immature rat Sertoli cells the polarity of ABP secretion, but not that of transferrin, may be regulated by pachytene spermatocytes (and possibly other germ cells), and that this process is FSH-dependent.
利用双室培养室研究了生殖细胞（大于85%的粗线期精母细胞）对未成熟大鼠支持细胞雄激素结合蛋白（ABP）和转铁蛋白的向量分泌的影响。单独培养支持细胞的对照培养物中，分泌到外室和内室的ABP比例为1.9，且不受促卵泡激素（FSH）或睾酮的影响。在FSH存在的情况下，将支持细胞与生殖细胞直接共培养可降低该比例，共培养2天后降低最为明显（降至0.7）。如果生殖细胞不与支持细胞单层直接接触，则未观察到这种效应。单独培养支持细胞时，转铁蛋白的外室与内室比例为1.6，与ABP不同的是，即使在添加生殖细胞且存在FSH的情况下，转铁蛋白比例也不受显著影响。得出的结论是，在未成熟大鼠支持细胞中，ABP分泌的极性（而非转铁蛋白的极性）可能受粗线期精母细胞（可能还有其他生殖细胞）调节，且该过程依赖于FSH。
