甘露聚糖-丝裂霉素C偶联物对移行细胞癌和正常小鼠成纤维细胞的抑制及凋亡作用

Inhibitory and Apoptotic Effects of Mannan-Mitomycin C Conjugate Against Transitional Cell Carcinoma and Normal Mouse Fibroblasts.

作者信息

Kabiri Ladan, Irani Shiva, Nikpoor Amin Reza, Tavakkol Afshari Jalil

机构信息

Department of biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Department of Immunogenetic, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences.

出版信息

Rep Biochem Mol Biol. 2019 Apr;8(1):63-71.

PMID:31334290

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6590937/

Abstract

BACKGROUND

Many studies have shown the anticancer effects of mannan and mitomycin C on tumor cells. In this regard, the aim of this study was to investigate the inhibitory and apoptotic effects of a mannan-mitomycin-C conjugate on transitional cell carcinoma (TCC) and normal mouse L929 fibroblast cells.

METHODS

The conjugate was synthesized according to previous studies. Both cell lines were cultured and the cytotoxic and apoptotic effects of the compounds in different concentrations were assessed using MTT and flow cytometry, respectively. The mannan-mitomycin C conjugate inhibited proliferation of both cell lines in time and concentration -dependent manners.

RESULTS

The conjugate inhibited TCC cell proliferation more than that of L929 cells. Mitomycin C alone inhibited proliferation of both cell lines in both time and concentration -dependent manners, and the effect was greater on L929 than on TCC cells. Mannan had a relatively low inhibitory effect on TCC and no significant effect on L929 cells. The percentage of apoptosis was greater in TCCs than in L929 cells at the highest concentration of conjugate. Mitomycin C induced apoptosis more extensively in L929 cells than in TCC cells at 25 and 400 μg/ml. The effect of mannan was similar on both cell lines.

CONCLUSION

The mannan-mitomycin C conjugate has greater inhibitory and apoptotic effects on TCC than on L929 cells and may inhibit TCC.

摘要

背景

许多研究已表明甘露聚糖和丝裂霉素C对肿瘤细胞具有抗癌作用。在这方面，本研究的目的是探讨甘露聚糖 - 丝裂霉素C缀合物对移行细胞癌（TCC）和正常小鼠L929成纤维细胞的抑制和凋亡作用。

方法

根据先前的研究合成缀合物。培养两种细胞系，并分别使用MTT和流式细胞术评估不同浓度化合物的细胞毒性和凋亡作用。甘露聚糖 - 丝裂霉素C缀合物以时间和浓度依赖性方式抑制两种细胞系的增殖。

结果

该缀合物对TCC细胞增殖的抑制作用大于对L929细胞的抑制作用。单独的丝裂霉素C以时间和浓度依赖性方式抑制两种细胞系的增殖，且对L929细胞的作用大于对TCC细胞的作用。甘露聚糖对TCC的抑制作用相对较低，对L929细胞无显著作用。在缀合物最高浓度下，TCC细胞的凋亡百分比高于L929细胞。在25和400μg/ml时，丝裂霉素C在L929细胞中诱导的凋亡比在TCC细胞中更广泛。甘露聚糖对两种细胞系的作用相似。