College of Biology and Environmental Engineering , Zhejiang Shuren University , Hangzhou 310015 , China.
Zhejiang Academy of Agricultural Sciences , Food Science Institute, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Key Laboratory of China Light Industry , Hangzhou 310021 , China.
J Agric Food Chem. 2019 Aug 14;67(32):8919-8925. doi: 10.1021/acs.jafc.9b03800. Epub 2019 Aug 2.
Histone deacetylase (HDAC) performs important functions in plant growth and development, including fruit ripening. As a complex biological process, fruit ripening involves the histone acetylation modification of ripening-associated genes. Histone deacetylase genes () have been well studied in and rice, but the biological functions of s in papaya are poorly understood. In the present work, three , belonging to the RPD3/HDA1 subfamily, were identified from papaya and named as , , and . and were induced by propylene, while was propylene-repressed. Moreover, CpHDA3 protein could physically interact with CpERF9 and enhance the transcriptional repression activities of CpERF9 to downstream genes , and . Histone acetylation levels of and were increased during fruit ripening. Taken together, these results suggested that CpERF9 recruits CpHDA3 to form a histone deacetylase repressor complex to mediate pectin methylesterase and polygalacturonase genes expression during papaya fruit ripening and softening.
组蛋白去乙酰化酶 (HDAC) 在植物生长发育中发挥着重要作用，包括果实成熟。作为一个复杂的生物学过程，果实成熟涉及到与成熟相关基因的组蛋白乙酰化修饰。在 和水稻中，HDAC 基因已得到很好的研究，但在木瓜中，s 的生物学功能还知之甚少。本研究从木瓜中鉴定出三个属于 RPD3/HDA1 亚家族的 ，并分别命名为 、 、 。 和 受丙烯诱导，而 受丙烯抑制。此外，CpHDA3 蛋白可以与 CpERF9 发生物理相互作用，并增强 CpERF9 对下游基因 、 和 的转录抑制活性。在果实成熟过程中， 和 的组蛋白乙酰化水平增加。综上所述，这些结果表明，CpERF9 招募 CpHDA3 形成组蛋白去乙酰化酶抑制复合物，在木瓜果实成熟和软化过程中调节果胶甲酯酶和多聚半乳糖醛酸酶基因的表达。
