Department of Chemistry, Faculty of Science, Çankırı Karatekin University, Çankırı, Turkey.
Department of Biology, Faculty of Arts and Sciences, Bingöl University, Bingöl, Turkey.
Nat Prod Res. 2021 Jun;35(11):1919-1922. doi: 10.1080/14786419.2019.1644507. Epub 2019 Jul 23.
It is aimed to investigate the cytotoxic effects of the various extracts from the leaf and seed of on the growth human cervical adenocarcinoma (HeLa) cells by xCELLigence method and to isolate the cytotoxic constituents. The results showed that subfractions 2 and 3 exhibited remarkable inhibitory effect against HeLa (IC < 10 µg/mL). The chloroform extract of leaf displayed the highest cytotoxic effect on HeLa cells (IC < 50 µg/mL) and was therefore subjected to a bioassay-guided multistep separation procedure. The pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1 D-, 2 D-NMR. In addition to cytotoxic effects of the isolated constituents, their antioxidant activities were also studied. On the other hand, subfraction 4 exhibited the highest DPPH radical scavenging activity (IC = 0.76 ± 0.03 mg/mL). β-sitosterol-3-O-β-d-glucopyranoside was isolated for the first time from this plant and three compounds from the bioactive subfractions were identified.
采用 xCELLigence 法研究不同叶和种子提取物对人宫颈腺癌（HeLa）细胞生长的细胞毒性作用，并分离具有细胞毒性的成分。结果表明，馏分 2 和 3 对 HeLa 细胞表现出显著的抑制作用（IC < 10 µg/mL）。叶的氯仿提取物对 HeLa 细胞显示出最高的细胞毒性作用（IC < 50 µg/mL），因此进行了基于生物测定的多步分离程序。通过光谱和质谱分析，包括 1D-NMR 和 2D-NMR，鉴定出纯化合物。除了分离成分的细胞毒性作用外，还研究了它们的抗氧化活性。另一方面，馏分 4 对 DPPH 自由基的清除活性最高（IC = 0.76 ± 0.03 mg/mL）。β-谷甾醇-3-O-β-D-吡喃葡萄糖苷首次从该植物中分离得到，从生物活性馏分中鉴定出三种化合物。
