Murphy J R, Williams D P, Bacha P, Bishai W, Waters C, Strom T B
Evans Department of Clinical Research, University Hospital, Boston, MA 02118.
J Recept Res. 1988;8(1-4):467-80. doi: 10.3109/10799898809049005.
We have genetically replaced the diphtheria toxin receptor binding domain with a DNA insert encoding the T-cell growth factor interleukin-2 (IL-2). The toxin-related IL-2 fusion gene encodes a 70,586 dalton protein, pro-IL-2-toxin. The mature form of IL-2-toxin is exported to the periplasmic space of recombinant Escherichia coli and has a molecular weight of 68,086. IL-2-toxin has been partially purified from periplasmic extracts of recombinant E. coli, and it is shown to contain immunologic determinants intrinsic to both its diphtheria toxin and IL-2 components. The chimeric toxin is targeted toward IL-2 receptor bearing T-cells in vitro.
我们已通过基因手段将白喉毒素受体结合域替换为编码T细胞生长因子白细胞介素-2（IL-2）的DNA插入片段。毒素相关的IL-2融合基因编码一种70,586道尔顿的蛋白质，即前体IL-2-毒素。IL-2-毒素的成熟形式被分泌到重组大肠杆菌的周质空间中，分子量为68,086。IL-2-毒素已从重组大肠杆菌的周质提取物中部分纯化，并且显示其含有白喉毒素和IL-2成分所固有的免疫决定簇。这种嵌合毒素在体外靶向带有IL-2受体的T细胞。
