1 College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, Shaanxi, 710119, People's Republic of China.
2 College of Biomedicine and Food Engineering, Shangluo University, Shangluo, 726000, People's Republic of China.
J Food Prot. 2019 Aug;82(8):1390-1397. doi: 10.4315/0362-028X.JFP-18-496.
Patulin contamination is a serious issue that restricts the development of the global fruit processing industry. Yeasts adsorb patulin more effectively than other microbial adsorbents, and this adsorption process depends mainly on the function of the cell wall. The present study examined the effect of exogenous calcium, in concentrations ranging from 0 to 1 mol/L, on cell wall structure and on patulin adsorption capability; the patulin adsorption capability of yeast was found to strengthen with an increase in exogenous calcium concentrations from 1 × 10 to 1 × 10 mol/L. Moreover, yeast cell wall thickness, β-1,3-glucan content, and the activities of the key catalytic enzymes β-1,3-glucanase and β-1,3-glycosyl transferase were all increased within this range. The results indicate that exogenous calcium activates key enzymes that are crucial for cell wall network formation and patulin adsorption capability.
棒曲霉素污染是限制全球水果加工业发展的一个严重问题。与其他微生物吸附剂相比,酵母更有效地吸附棒曲霉素,而这种吸附过程主要依赖于细胞壁的功能。本研究考察了外源钙浓度在 0 至 1 mol/L 范围内对细胞壁结构和棒曲霉素吸附能力的影响,结果发现随着外源钙浓度从 1×10 增加到 1×10 mol/L,酵母的棒曲霉素吸附能力增强。此外,在这个范围内,酵母细胞壁厚度、β-1,3-葡聚糖含量和关键催化酶β-1,3-葡聚糖酶和β-1,3-糖苷转移酶的活性均增加。研究结果表明,外源钙激活了细胞壁网络形成和棒曲霉素吸附能力的关键酶。