D-甘露醇和2-O-甲基手性肌醇（奎布糖醇）在柔嫩艾美耳球虫中的鉴定及其可能作用

Identification and possible role of D-mannitol and 2-O-methyl-chiro-inositol (quebrachitol) in Eimeria tenella.

作者信息

Schmatz D M, Arison B H, Dashkevicz M P, Liesch J M, Turner M J

机构信息

Department of Biochemical Parasitology, Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065.

出版信息

Mol Biochem Parasitol. 1988 May;29(1):29-36. doi: 10.1016/0166-6851(88)90116-8.

DOI:10.1016/0166-6851(88)90116-8

PMID:3133561

Abstract

Analysis of polyol extracts from various stages of Eimeria tenella has revealed the presence of mannitol and 2-O-methyl-chiro-inositol (quebrachitol). Previously, both compounds had been found almost exclusively in plants, and in the case of mannitol in a few species of bacteria. Identification was achieved by various analytical techniques including nuclear magnetic resonance (NMR), capillary gas-liquid chromatography (GLC), and GLC-mass spectrometry. Unsporulated oocysts contain a high level of mannitol (300 mM) which diminished during sporulation to 10 mM in sporulated oocysts.

摘要

对柔嫩艾美耳球虫不同阶段的多元醇提取物进行分析后发现了甘露醇和2-O-甲基手性肌醇（肌醇）的存在。此前，这两种化合物几乎只在植物中被发现，而甘露醇只在少数几种细菌中被发现。通过包括核磁共振（NMR）、毛细管气液色谱法（GLC）和GLC-质谱法在内的各种分析技术实现了鉴定。未孢子化的卵囊含有高水平的甘露醇（300 mM），在孢子化过程中减少到孢子化卵囊中的10 mM。