基于乙酰化碎米淀粉纳米晶的疏水性薄膜的制备及性质研究及其在缓慢蛋白释放中的应用。
Preparation and properties of hydrophobic films based on acetylated broken-rice starch nanocrystals for slow protein delivery.
机构信息
National Engineering Laboratory for Deep Processing of Rice and Byproducts, Central South University of Forestry & Technology, Changsha 410004, China; College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China.
College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha 410004, China.
出版信息
Int J Biol Macromol. 2019 Oct 1;138:556-564. doi: 10.1016/j.ijbiomac.2019.07.121. Epub 2019 Jul 20.
Native and acetylated broken-rice starches (nanocrystals) with different degrees of substitution (DS) and their corresponding films were individually prepared, and the drug release profiles, weight loss, solubility and dispersion and surface morphology were comparatively studied. Bovine serum albumin (BSA) was used as a model drug. Acetylated native starch (ANS) DS 2.58, acetylated starch nanocrystals (ASN) DS 0.98, ASN DS 1.86, and ASN DS 2.72 were observed to be very soluble in chloroform. BSA was released rapidly from the native rice starch (NS) and ANS DS 2.58 films. ASN with high DS significantly slowed down the release of BSA from films, the percentages of BSA released from film ASN DS 2.72 only reached to 13% after 3.5 weeks release, and the release data followed Korsmeyer-Peppas equation. Further studies reveal that the particle size of ASN DS 2.72 was smallest, and the weight loss of ASN DS 2.72 film was lowest. The results demonstrate that acetylation and nanometer particle form of rice starch film can effectively retard protein drug release, and the prepared films based on ASN with high DS from broken rice may be suitable for the controlled protein delivery.
天然和乙酰化碎米淀粉（纳米晶体）具有不同取代度（DS），分别制备了它们各自的薄膜，并对其药物释放曲线、失重、溶解度和分散度以及表面形貌进行了比较研究。牛血清白蛋白（BSA）被用作模型药物。乙酰化天然淀粉（ANS）DS2.58、乙酰化淀粉纳米晶体（ASN）DS0.98、ASN DS1.86 和 ASN DS2.72 被观察到在氯仿中非常可溶。BSA 从天然大米淀粉（NS）和 ANS DS2.58 薄膜中快速释放。高 DS 的 ASN 显著减缓了 BSA 从薄膜中的释放，BSA 从薄膜 ASN DS2.72 中的释放百分比仅在 3.5 周释放后达到 13%，并且释放数据符合 Korsmeyer-Peppas 方程。进一步的研究表明，ASN DS2.72 的粒径最小，ASN DS2.72 薄膜的失重率最低。结果表明，大米淀粉薄膜的乙酰化和纳米颗粒形式可以有效延缓蛋白质药物的释放，并且基于高 DS 的 ASN 制备的碎米薄膜可能适合于控制蛋白质的传递。
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