Department of R&D, Decode Genomics Incorporation, Nanjing, 210000, China; Department of R&D, AGCU ScienTech Incorporation, Wuxi, 214174, China.
Department of R&D, Decode Genomics Incorporation, Nanjing, 210000, China.
Mol Cell Probes. 2019 Aug;46:101424. doi: 10.1016/j.mcp.2019.101424. Epub 2019 Jul 20.
At present, approaches to hybrid capture sequencing have many limitations, from their significant complexity and labor requirements, to their low enrichment efficiency, resulting in their limited utilization. In an effort to overcome these drawbacks, we have developed a novel method that relied upon direct genomic DNA hybridization and single-stranded DNA library preparation. Using this novel protocol, we were able to achieve a targeting efficiency as high as 75%, and we found this approach to overall be an efficient and simple approach to DNA library construction.
目前，杂交捕获测序方法存在许多局限性，从其显著的复杂性和劳动力需求，到其低富集效率，导致其利用受限。为了克服这些缺点，我们开发了一种新的方法，该方法依赖于直接基因组 DNA 杂交和单链 DNA 文库制备。使用这种新方案，我们能够实现高达 75%的靶向效率，并且我们发现这种方法总体上是一种高效且简单的 DNA 文库构建方法。
