Shah Assar Ali, Khan Irfan Ullah, Sahibzada Fayaz Ahmed, Tauseef Isfahan, Kalsoom Umm-E-, Sultana Nighat
National Forage Breeding Innovation Base, Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural University, Nanjing, China.
Reprod Domest Anim. 2019 Oct;54(10):1348-1356. doi: 10.1111/rda.13521. Epub 2019 Sep 12.
The aim of the current study was to evaluate the effect of dietary supplementation of Pinus ponderosa leaves (pine leaves) and α-tocopherol acetate (vitamin E) powder on male reproductive system, serum metabolites and carcass characteristics of Japanese quails. A total of 360-day-old male quails were purchased from the open market and kept at poultry shed for ninety-four days. After ten days of adaptation, all quails were randomly assigned into 4 groups, control (IC); supplemented with α-tocopherol acetate (IE) at the rate of 150 mg/L; Pinus ponderosa leaves (IP) at the rate of 150 mg/L; and 70 mg α-tocopherol acetate and 70 mg Pinus ponderosa leaves (IEP). Pinus ponderosa leaves and α-tocopherol acetate supplementation had not significantly (p > .05) effected on final body weight gain, feed intake and feed conversion ratio of quails. The high-density lipoprotein cholesterol (HDLC) and total cholesterol (TC) were significantly (p > .05) affected by IE and IP groups as compared to IC and IEP groups. Triglyceride (TG), glutathione peroxidase (GPx) and superoxide dismutase (SOD) significantly (p < .05) increased in all treatment groups except for the IC group. Aspartate transaminase (AST) significantly (p > .05) decreased in treatment groups as compared to control group. Overall, the mineral levels significantly (p < .05) increased in treatment groups as compared to control. Cloacal gland index values, the quantity of foam production and testis weight were significantly (p < .05) increased in treatment groups. It was concluded that the supplementation of Pinus ponderosa leaves and α-tocopherol acetate improved the testis weight, foam production, serum antioxidant enzymes and mineral level especially zinc in Japanese quail considered an indicative characteristic of higher sperm production rate and improved sexual activity. Further, higher gametogenesis rate, sperm production or reproductive behaviour including different hormonal level will be analysed in future study.
本研究的目的是评估日粮中添加黄松叶（松叶）和α-生育酚醋酸酯（维生素E）粉对日本鹌鹑雄性生殖系统、血清代谢物和胴体特性的影响。总共从公开市场购买了360日龄雄性鹌鹑，并在禽舍饲养94天。适应10天后，将所有鹌鹑随机分为4组，对照组（IC）；以150 mg/L的剂量补充α-生育酚醋酸酯（IE）；以150 mg/L的剂量补充黄松叶（IP）；以及70 mg α-生育酚醋酸酯和70 mg黄松叶（IEP）。补充黄松叶和α-生育酚醋酸酯对鹌鹑的最终体重增加、采食量和饲料转化率没有显著影响（p>0.05）。与IC组和IEP组相比，IE组和IP组对高密度脂蛋白胆固醇（HDLC）和总胆固醇（TC）有显著影响（p>0.05）。除IC组外，所有处理组的甘油三酯（TG）、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）均显著增加（p<0.05）。与对照组相比，处理组的天冬氨酸转氨酶（AST）显著降低（p>0.05）。总体而言，与对照组相比，处理组的矿物质水平显著增加（p<0.05）。处理组的泄殖腔腺指数值、泡沫产生量和睾丸重量显著增加（p<0.05）。得出的结论是，补充黄松叶和α-生育酚醋酸酯可改善日本鹌鹑的睾丸重量、泡沫产生、血清抗氧化酶和矿物质水平，尤其是锌，这被认为是精子产生率更高和性活动改善的指示性特征。此外，未来的研究将分析更高的配子发生率、精子产生或包括不同激素水平在内的生殖行为。
