Phytopathology and Agricultural Zoology Research Unit, Plant Protection Department, Faculty of Agronomy and Agricultural Sciences, University of Dschang, Dschang, Republic of Cameroon.
Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi 30709, Kenya.
Toxins (Basel). 2019 Jul 22;11(7):430. doi: 10.3390/toxins11070430.
The prevalence and concentrations of three major mycotoxins, total aflatoxin (AFs), fumonisin (F), and zearalenone (ZEN), were determined on seven edible non-timber forest products (ENTFP) in Cameroon. A total of 210 samples consiting of 30 samples from each ENTFP commodity was collected from farmers and local markets in three agroecological zones of Cameroon and analyzed for moisture content and mycotoxins. Mycotoxins were analyzed using commercial enzyme-linked immunosorbent assay (ELISA) kits and results were validated using the VICAM fluorometric method. The European Union regulation of mycotoxins for human consumption (N°1881/2006) was adopted as reference. The moisture content of samples varied from 5.0% to 22.6%. Aflatoxin contamination was detected in 84.3% samples and only 5.7% exceeded the legal limit (10 ppb). Similarly, 53% of samples were contaminated with fumonisin and 5% of samples exceeded the legal limit (1000 ppb). Zearalenone contamination was detected in 92% of samples and 21% of samples exceeded the legal limit (100 ppb). This is the first report on mycotoxin contamination of ENTFP in the Congo Basin forest. The findings of this study will form a basis for educating farmers and other stakeholders of ENTFP values chain on mycotoxins and mycotoxin mitigation measures to produce safe ENTFP for local and international markets.
对喀麦隆七种食用非木材林产品（ENTFP）中的三种主要霉菌毒素（总黄曲霉毒素（AFs）、伏马菌素（F）和玉米赤霉烯酮（ZEN））的流行情况和浓度进行了测定。从喀麦隆三个农业生态区的农民和当地市场共收集了 210 个样本，每个 ENTIF 商品 30 个样本，用于检测水分含量和霉菌毒素。使用商业酶联免疫吸附测定（ELISA）试剂盒分析霉菌毒素，并用 VICAM 荧光法验证结果。采用欧盟人类消费用霉菌毒素法规（N°1881/2006）作为参考。样品的水分含量从 5.0%到 22.6%不等。在 84.3%的样品中检测到黄曲霉毒素污染，只有 5.7%超过法定限量（10 ppb）。同样，53%的样品受到伏马菌素的污染，5%的样品超过法定限量（1000 ppb）。在 92%的样品中检测到玉米赤霉烯酮污染，21%的样品超过法定限量（100 ppb）。这是刚果盆地森林中首次报告食用非木材林产品受霉菌毒素污染的情况。本研究的结果将为教育农民和食用非木材林产品价值链的其他利益相关者提供霉菌毒素和霉菌毒素缓解措施的基础，以生产适合当地和国际市场的安全食用非木材林产品。
