School of Chinese Materia Medica, Beijing University of Chinese Medicine, Yangguang South Avenue, Fangshan District, Beijing 102488, China.
Yifan Pharmaceutical Co., Ltd., Lin'an 311300, China.
Molecules. 2019 Jul 14;24(14):2561. doi: 10.3390/molecules24142561.
Houpo Wenzhong Decoction (HWD) as a classical prescription has been widely used for hundreds of years. However, the quality of HWDs is difficult to control because of its herb materials from different regions. It is a new idea to use HPLC fingerprints, LTQ-ESI-Orbitrap-MS, and network pharmacology in combination to screen common components (CCs) as potential quality control indicators. In this paper, the fingerprints of HWDs were established, which were used to determine HWDs compounded from different sources of traditional Chinese medicines (TCMs). Through the similarity analysis, 45 CCs were selected. UHPLC-LTQ-ESI-Orbitrap-MS was used to carry out the chemical composition analysis of HWD. Seventy-three chemical constituents were distinguished, and 30 CCs were identified. Through network pharmacology, networks of candidate CCs, diseases, and candidate targets were constructed. Finally, four CCs were screened as potential active ingredient markers of HWD, and a method for content determination of these four components was established. A rapid, reasonable, and effective method for quality evaluation and control of HWDs was established. It provides a reference for the further development and research of HWDs and a new way of thinking for the research of other Chinese medicine prescriptions.
厚朴温中汤（HWD）作为一种经典方剂，已广泛应用数百年。然而，由于其药材来源于不同地区，HWD 的质量难以控制。本文首次采用 HPLC 指纹图谱、LTQ-ESI-Orbitrap-MS 和网络药理学相结合的方法，筛选出共同成分（CCs）作为潜在的质量控制指标。本研究建立了 HWD 的指纹图谱，用于确定来源于不同中药（TCM）来源的 HWD 化合物。通过相似度分析，选择了 45 个 CCs。采用 UHPLC-LTQ-ESI-Orbitrap-MS 对 HWD 的化学成分进行分析。共鉴别出 73 种化学成分，鉴定出 30 个 CCs。通过网络药理学，构建了候选 CCs、疾病和候选靶点的网络。最后，筛选出 4 个 CCs 作为 HWD 的潜在活性成分标志物，并建立了这 4 个成分的含量测定方法。建立了一种快速、合理、有效的 HWD 质量评价和控制方法，为 HWD 的进一步开发和研究提供了参考，也为其他中药方剂的研究提供了新的思路。
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