选择最优的穿刺针用于诱导大鼠椎间盘退变模型。
Selection of the Optimal Puncture Needle for Induction of a Rat Intervertebral Disc Degeneration Model.
机构信息
Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China; Department of Orthopaedic Surgery, Suzhou Dushuhu Public Hospital, Suzhou, Jiangsu, China.
Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
出版信息
Pain Physician. 2019 Jul;22(4):353-360.
BACKGROUND
The incidence of intervertebral disc (IVD) degeneration has increased in recent years. A simple, reliable, and reproducible animal model is critical for understanding the underlying mechanisms of IVD degeneration. The caudal discs of rats have been proposed as a common puncture model in which to induce IVD degeneration. However, there is still no consensus on the size of needle to be used.
OBJECTIVES
The present study aimed to identify the appropriate needle size to establish an IVD degeneration model.
STUDY DESIGN
A randomized, experimental trial.
SETTING
Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, China.
METHODS
Validity was verified by magnetic resonance imaging (MRI) and histology.
RESULTS
From T2-weighted MRI imaging and histological examination, the IVD punctured by the 16-gauge needle degenerated acutely one week after the operation, whereas the 26-gauge needle puncture did no harm to the IVD. An 18-gauge needle showed a progressive degeneration in IVD.
LIMITATIONS
The observation period was not very long (4 weeks).
CONCLUSIONS
An 18-gauge needle can be used to induce IVD degeneration in rats. Therefore, an 18-gauge needle is the optimal selection to establish the degenerative IVD model on rats, whereas the 26-gauge needle failed to cause IVD degeneration. Thus, to study the prevention and treatment of IVD degeneration, a 26-gauge needle can be used for IVD injection of growth factors, plasmids, and drugs. A 16-gauge needle may be used to induce acute disc injury, but not IVD degeneration.
KEY WORDS
Low back pain, degenerative intervertebral disc, animal model, puncture needle, rat model, optimal choice.
背景
近年来，椎间盘（IVD）退变的发病率有所增加。对于理解 IVD 退变的潜在机制，一种简单、可靠且可重复的动物模型至关重要。大鼠尾椎椎间盘已被提议作为一种常见的穿刺模型，用于诱导 IVD 退变。然而，对于使用的针的大小，目前仍没有共识。
目的
本研究旨在确定合适的针的大小，以建立 IVD 退变模型。
研究设计
随机、实验性试验。
设置
中国苏州大学第一附属医院骨科。
方法
通过磁共振成像（MRI）和组织学验证有效性。
结果
从 T2 加权 MRI 成像和组织学检查来看，16 号针穿刺的 IVD 在手术后一周内急性退变，而 26 号针穿刺对 IVD 没有损害。18 号针显示 IVD 进行性退变。
局限性
观察期不是很长（4 周）。
结论
18 号针可用于诱导大鼠 IVD 退变。因此，18 号针是建立大鼠退变 IVD 模型的最佳选择，而 26 号针不能引起 IVD 退变。因此，为了研究 IVD 退变的预防和治疗，26 号针可用于 IVD 注射生长因子、质粒和药物。16 号针可用于诱导急性椎间盘损伤，但不能用于 IVD 退变。
关键词
下腰痛；退行性椎间盘；动物模型；穿刺针；大鼠模型；最佳选择。
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