HLA A32 与 HIV 获得性感染相关，而 B44 和 B*53 与围生期暴露婴儿的 HIV 获得性感染保护相关。

HLA A32 is associated to HIV acquisition while B44 and B*53 are associated with protection against HIV acquisition in perinatally exposed infants.

机构信息

Faculty of Science, University of Dschang, P.O. Box 56, Dschang, Cameroon.

Chantal BIYA International Reference Centre, P.O. Box 3077, Yaounde, Cameroon.

出版信息

BMC Pediatr. 2019 Jul 23;19(1):249. doi: 10.1186/s12887-019-1620-6.

DOI:10.1186/s12887-019-1620-6

PMID:31337377

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6647251/

Abstract

BACKGROUND

Human leukocyte antigen (HLA) molecules play a key role in the cellular immune system. They may be determinants of mother-to-child transmission which is the driving force in pediatric HIV infection. We intended to look at the impact of the distribution of these polymorphic HLA genes in the mother-to-child transmission (MTCT) of HIV in Cameroon.

METHODS

A total of 156 mother-baby pairs were enrolled in three hospitals of Yaounde, capital of Cameroon. After the extraction of the DNA from blood samples using the Qiagen Kit as per manufacturer' instructions, the polymorphism of the HLA class 1 ABC was determined using the PCR- sequence specific primers assay.

RESULTS

The distribution of HLA class 1 revealed that none of the allele studied was associated with transmitters or non-transmitters, so was not implicated in transmission. The regression analysis showed that HLA A32 [OR 0.062 (CI; 0.0075 to 0.51)] is associated with HIV acquisition while HLA B44 [OR 0.47 (CI; 0.21 to 1.14)] and HLA B53 [OR; 0.14 (CI; 0.018 to 1.22)] were implicated in reducing the acquisition of HIV by infants. The homozygosity of locus C [OR 6.99 (CI; 1.81 to 26.88), p = 0.0027] was found as a risk factor for the acquisition, while the A32-B*44 haplotype [OR 10.1 (CI 1.17 to 87.87), p = 0.03] was a risk factor for the transmission.

CONCLUSION

This study has found that HLA A32, B44 and B53 have an impact in MTCT outcomes. The homozygosity of locus C and the A32-B*44 haplotype were risk factors for acquisition and transmission respectively.

摘要

背景

人类白细胞抗原 (HLA) 分子在细胞免疫系统中发挥关键作用。它们可能是母婴传播的决定因素，而母婴传播是儿科 HIV 感染的驱动力。我们旨在研究 HLA 多态性基因在喀麦隆母婴传播 (MTCT) 中对 HIV 的影响。

方法

共纳入喀麦隆首都雅温得的三家医院的 156 对母婴。按照制造商的说明，使用 Qiagen 试剂盒从血样中提取 DNA 后，使用聚合酶链反应-序列特异性引物测定法确定 HLA Ⅰ类 ABC 的多态性。

结果

HLA Ⅰ类的分布表明，所研究的等位基因均与传播者或非传播者无关，因此与传播无关。回归分析表明，HLA A32 [OR 0.062 (CI; 0.0075 至 0.51)] 与 HIV 获得有关，而 HLA B44 [OR 0.47 (CI; 0.21 至 1.14)] 和 HLA B53 [OR; 0.14 (CI; 0.018 至 1.22)] 则与婴儿感染 HIV 减少有关。发现 C 座的纯合性 [OR 6.99 (CI; 1.81 至 26.88), p = 0.0027] 是获得的危险因素，而 A32-B*44 单倍型 [OR 10.1 (CI 1.17 至 87.87), p = 0.03] 是传播的危险因素。