UOC of Pathology, Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, Latina, Italy.
UOC of Pathology, Department of Medical-Surgical Sciences and Bio-Technologies, Sapienza University of Rome, Latina, Italy.
Pathol Res Pract. 2019 Oct;215(10):152534. doi: 10.1016/j.prp.2019.152534. Epub 2019 Jul 12.
In this study we investigated the role of KMT2C (a chromatin-modifying and remodelling protein) in osteosarcoma progression through cell migration and invasion assays in osteosarcoma primary and metastatic cell lines. Wound healing and transwell assays were used to detect changes of cell migration and matrigel assay was used to evaluate changes of cell invasion in primary and metastatic osteosarcoma cell lines after KMT2C siRNA transfection. We found that primary osteosarcoma cell lines showed the highest capacity of migration before mRNA KMT2C silencing and the highest capacity of invasion after mRNA KMT2C silencing; on the contrary, osteosarcoma metastatic cell line showed the highest capacity of migration after mRNA KMT2C silencing and the highest capacity of invasion before mRNA KMT2C silencing. Our study supports data in favour of selective enhancer changes, KMT2C-mediated, in metastatic osteosarcoma probably due to the different microenvironment between primary and metastatic sites.
在这项研究中，我们通过在骨肉瘤原代和转移性细胞系中进行细胞迁移和侵袭实验，研究了 KMT2C（一种染色质修饰和重塑蛋白）在骨肉瘤进展中的作用。我们使用划痕愈合和 Transwell 实验检测 KMT2C siRNA 转染后骨肉瘤原代和转移性细胞系中细胞迁移的变化，用 Matrigel 实验评估细胞侵袭的变化。我们发现，在 mRNA KMT2C 沉默之前，原代骨肉瘤细胞系显示出最高的迁移能力，而在 mRNA KMT2C 沉默之后，原代骨肉瘤细胞系显示出最高的侵袭能力；相反，骨肉瘤转移细胞系在 mRNA KMT2C 沉默后显示出最高的迁移能力，而在 mRNA KMT2C 沉默前显示出最高的侵袭能力。我们的研究支持了选择性增强子变化的数据，即 KMT2C 介导的转移性骨肉瘤，可能是由于原发部位和转移部位之间的微环境不同。
