Adetula Adeyinka Abiola, Azmal Syed Ali, Sun Chenghao, Elokil Abdelmotaleb, Li Shijun
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei, China.
Department of Livestock Services (DLS), Under the Ministry of Fisheries and Livestock (MOFL), Dhaka, Bangladesh.
PeerJ. 2019 Jul 12;7:e7282. doi: 10.7717/peerj.7282. eCollection 2019.
A previous genome-wide transcriptional analysis identified long non-coding RNA 8138.1 () as a candidate gene related to hen duration of the fertility (DF) trait. gene response to growth factor and reproductive system development suggests it has a vital role in reproduction. In this study, we investigated the gene sequence in a population of egg-laying hens. The sequence analysis of the gene containing about 1.6 k nucleotides (nt) was observed with four single nucleotide polymorphisms (SNPs) and 7 nt indel including r.4937159A > G; r.4937219T > C; r.4937258G > C; r.4937318C > G and g.4937319_4937325delinsTGTGTGG. Next, the genomic DNAs from laying hen populations were subjected to polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) to detect a region of 457 bp carrying r.4937159A > G substitution. Further inspection of the region containing r.4937159A > G mutation revealed three genotypes viz., AA, AG, and GG were observed with respective frequencies of 0.106, 0.607, and 0.287 in laying hen population 1 (P1) ( = 1, 042) and respective frequencies of 0.176, 0.708, and 0.116 in laying hen population 2 (P2) ( = 826). Moreover, to further examining the frequencies of r.4937159A > G genotypes in P1 and P2, and their additive and dominance effects; r.4937159A > G locus was significantly associated with DF-trait in both P1 and P2 (EN: the number of eggs, FN: the number of fertile eggs after a single AI), and DN (the number of days post-insemination until last fertile egg). In testing for additive and dominance effects, additive effect was significant ( < 0.05) in both P1 and P2 for DF-trait, and the dominance effect was significant ( < 0.05) for EN and FN traits, suggesting that r.4937159A > G polymorphism is a potential biomarker for DF-trait. However, the identified novel r.4937159A > G mutation and others require further investigation to confirm phenotypic causality and potential genetic relationships with reproductive traits. Overall, our findings suggest the significance of genetic variation in long non-coding RNAs may assist in future breeding programs to improve selection for prolonged DF-trait.
先前的全基因组转录分析确定长链非编码RNA 8138.1（）是与母鸡受精持续时间（DF）性状相关的候选基因。该基因对生长因子和生殖系统发育的反应表明它在繁殖中起着至关重要的作用。在本研究中，我们调查了蛋鸡群体中的该基因序列。对包含约1600个核苷酸（nt）的该基因进行序列分析，发现了4个单核苷酸多态性（SNP）和7个nt插入/缺失，包括r.4937159A>G；r.4937219T>C；r.4937258G>C；r.4937318C>G和g.4937319_4937325delinsTGTGTGG。接下来，对蛋鸡群体的基因组DNA进行聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析，以检测携带r.4937159A>G替换的457 bp区域。对包含r.4937159A>G突变的区域进行进一步检查，发现蛋鸡群体1（P1）（n = 1042）中观察到三种基因型，即AA、AG和GG，其频率分别为0.106、0.607和0.287，在蛋鸡群体2（P2）（n = 826）中频率分别为0.176、0.708和0.116。此外为了进一步研究P1和P2中r.4937159A>G基因型的频率及其加性和显性效应；r.4937159A>G位点在P1和P2中均与DF性状显著相关（EN：产蛋数，FN：单次人工授精后的受精卵数）以及DN（授精后直至最后一枚受精卵的天数）。在测试加性和显性效应时，P1和P2中DF性状的加性效应均显著（P<0.05），EN和FN性状的显性效应显著（P<0.05），表明r.4937159A>G多态性是DF性状的潜在生物标志物。然而，鉴定出的新的r.4937159A>G突变和其他突变需要进一步研究，以确认表型因果关系以及与生殖性状的潜在遗传关系。总体而言，我们的研究结果表明长链非编码RNA中遗传变异的重要性可能有助于未来的育种计划，以改善对延长DF性状的选择。
