Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, and Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CG Utrecht, The Netherlands.
Org Biomol Chem. 2019 Aug 7;17(31):7304-7308. doi: 10.1039/c9ob01368g.
Disialosyl globopentaosylceramide (DSGb5) is often expressed by renal cell carcinomas. To investigate properties of DSGb5, we have prepared its oligosaccharide moiety by chemically synthesizing Gb5 which was enzymatically sialylated using the mammalian sialyltransferases ST3Gal1 and ST6GalNAc5. Glycan microarray binding studies indicate that Siglec-7 does not recognize DSGb5, and preferentially binds Neu5Acα(2,8)Neu5Ac containing glycans.
双涎酸神经节苷脂五糖（DSGb5）通常在肾细胞癌中表达。为了研究 DSGb5 的性质，我们通过使用哺乳动物唾液酸转移酶 ST3Gal1 和 ST6GalNAc5 对 Gb5 进行酶涎酸化，从而化学合成了 DSGb5 的寡糖部分。糖基微阵列结合研究表明，Siglec-7 不识别 DSGb5，而是优先结合含有 Neu5Acα(2,8)Neu5Ac 的聚糖。
