State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Key Lab of Biopesticides and Chemical Biology, MOE , Fujian Agriculture and Forestry University , 350002 Fuzhou , Fujian , PR China.
Key Laboratory of Genetics, Breeding and Comprehensive Utilization of Crops, Ministry of Education, College of Crop Science , Fujian Agriculture and Forestry University , 350002 Fuzhou , Fujian , People's Republic of China.
J Agric Food Chem. 2019 Aug 14;67(32):8896-8904. doi: 10.1021/acs.jafc.9b01840. Epub 2019 Aug 6.
The mosquito is associated with the spread of many viral diseases in humans, including Dengue virus (DENVs), Yellow fever virus (YFV), Zika virus (ZIKV), and Chikungunya virus (CHIKV). (Bt) is widely used as a biopesticide, which produces Cry toxins for mosquito control. The Cry toxins bind mainly to important receptors, including alkaline phosphatase (ALP) and aminopeptidase-N (APN). This work investigated the function of a C-type lectin, CTLGA9, in in response to Cry toxins. Our results showed by far-western blot and ELISA methods that the CTLTGA9 protein interacted with brush border membrane vesicles (BBMVs) of larvae and with ALP1, APN, and Cry11Aa proteins. Furthermore, molecular docking showed overlapping binding sites in ALP1 and APN for binding to Cry11Aa and CTLGA9. The toxicity assays further demonstrated that CTLGA9 inhibited the larvicidal activity of Cry toxins. According to the results of molecular docking, CTLGA9 may compete with Cry11Aa for binding to ALP1 and APN receptors and thus decreases the mosquitocidal toxicity of Cry11Aa. Our results provide further insights into better understanding the mechanism of Cry toxins and help improve the Cry toxicity for mosquito control.
蚊子与许多人类病毒性疾病的传播有关，包括登革热病毒（DENV）、黄热病病毒（YFV）、寨卡病毒（ZIKV）和基孔肯雅热病毒（CHIKV）。苏云金芽孢杆菌（Bt）被广泛用作生物农药，它产生 Cry 毒素来控制蚊子。Cry 毒素主要与重要的受体结合，包括碱性磷酸酶（ALP）和氨肽酶-N（APN）。这项工作研究了 C 型凝集素 CTLGA9 在 中的功能，以响应 Cry 毒素。我们的结果通过 far-western blot 和 ELISA 方法表明，CTLGA9 蛋白与幼虫的刷状缘膜泡（BBMV）以及 ALP1、APN 和 Cry11Aa 蛋白相互作用。此外，分子对接显示 ALP1 和 APN 中存在重叠的结合位点，用于与 Cry11Aa 和 CTLGA9 结合。毒性测定进一步表明 CTLGA9 抑制了 Cry 毒素的杀虫活性。根据分子对接的结果，CTLGA9 可能与 Cry11Aa 竞争与 ALP1 和 APN 受体结合，从而降低 Cry11Aa 的杀蚊毒性。我们的结果提供了对 Cry 毒素作用机制的进一步了解，并有助于提高 Cry 毒性以控制蚊子。
