Argenzio R A, Henrikson C K, Liacos J A
Department of Anatomy, Physiological Sciences, and Radiology, School of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Am J Physiol. 1988 Jul;255(1 Pt 1):G62-71. doi: 10.1152/ajpgi.1988.255.1.G62.
Acute injury of the porcine colonic epithelium was induced in vivo with the bile salt, deoxycholate. A concentration of 15 mM for 30 min completely destroyed the surface epithelium and induced a marked increase in mucosal permeability to mannitol. The crypt epithelium however was not significantly affected. Within 8 min of recovery, the colonic surface was reepithelialized with flattened, migrating cells, and within 40 min, mucosal permeability to mannitol was normalized. In vitro studies showed that in these early stages of recovery, NaCl transport, short-circuit current, and resistance were markedly impaired, whereas the theophylline-induced secretory response remained intact. Recovery of absorptive function paralleled the transition from flattened to columnar surface epithelium and was complete within 2 h. Results suggest that 1) active migratory events play an important role in rapid restitution of an epithelial barrier, 2) active absorption of ions is much slower to recover, and 3) active secretory events are intact and probably originate in the crypt epithelium.
用胆盐脱氧胆酸盐在体内诱导猪结肠上皮的急性损伤。15 mM的浓度作用30分钟可完全破坏表面上皮，并导致黏膜对甘露醇的通透性显著增加。然而，隐窝上皮未受到明显影响。恢复8分钟内，结肠表面被扁平的迁移细胞重新上皮化，40分钟内，黏膜对甘露醇的通透性恢复正常。体外研究表明，在恢复的早期阶段，氯化钠转运、短路电流和电阻明显受损，而茶碱诱导的分泌反应保持完整。吸收功能的恢复与表面上皮从扁平到柱状的转变同步，并在2小时内完成。结果表明：1）活跃的迁移事件在上皮屏障的快速修复中起重要作用；2）离子的主动吸收恢复较慢；3）活跃的分泌事件是完整的，可能起源于隐窝上皮。
