Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
Graduate School of Infectious Diseases, Hokkaido University, Sapporo, Japan.
Vector Borne Zoonotic Dis. 2019 Nov;19(11):859-866. doi: 10.1089/vbz.2018.2429. Epub 2019 Jul 24.
We have reported high seroprevalence to Thailand orthohantavirus (THAIV) or THAIV-related orthohantavirus (TRHV) among patients with chronic kidney disease of unknown etiology in Girandurukotte, Sri Lanka. THAIV or TRHV infection is considered to be transmitted by rodent hosts in this area, but its reservoir rodents have not yet been identified. Hence, 116 rodents were captured, and seroprevalences were examined by indirect immunofluorescent antibody assay (immunofluorescence assay [IFA]) using antigens of THAIV strain Thai749-infected Vero E6 cells and recombinant nucleocapsid protein of THAIV expressed in Vero E6 cell. Molecular biological species identification of rodents was carried out by sequencing , , and mitochondrial genes. The majority (112/116) of the captured rodents were lineage Ib of black rats (). Among them, 19.6% (22/112) of the rats possessed antibodies against THAIV. Also, a lesser bandicoot rat (), which belongs to the Sri Lankan endemic genetic lineage, was seropositive (1/1). Two and one sp. were seronegative. Rodent sera showed less cross-reactivities to antigens of Vero E6 cells infected with Hantaan orthohantavirus (HTNV), Seoul orthohantavirus (SEOV), and Puumala orthohantavirus (PUUV) in IFA. These results suggest that the hantavirus present in rodents in Sri Lanka is related to THAIV or TRHV rather than to SEOV, HTNV, or PUUV. However, it might be serologically distinct from the prototype THAIV strain, Thai749, used in this study. This study revealed that black rats and lesser bandicoot rats belonging to Sri Lankan endemic lineages are possible reservoirs for THAIV or TRHV in Girandurukotte. Further multiple geographical studies are needed to confirm the THAIV or TRHV reservoir status of black and lesser bandicoot rats in Sri Lanka.
我们曾报道，在斯里兰卡 Girandurukotte 地区病因不明的慢性肾脏病患者中，泰国汉坦病毒（THAIV）或 THAIV 相关汉坦病毒（TRHV）的血清阳性率较高。在该地区，THAIV 或 TRHV 感染被认为是由啮齿动物宿主传播的，但尚未确定其储存宿主啮齿动物。因此，我们捕获了 116 只啮齿动物，并通过间接免疫荧光抗体检测法（IFA），使用感染了 THAIV 株 Thai749 的 Vero E6 细胞和在 Vero E6 细胞中表达的 THAIV 重组核衣壳蛋白作为抗原，检测血清阳性率。通过测序 、 和线粒体 基因对啮齿动物的分子生物学种进行鉴定。捕获的啮齿动物中，大多数（112/116）为黑线姬鼠（）的 Ib 谱系。其中，22/112（19.6%）的大鼠具有针对 THAIV 的抗体。此外，一只斯里兰卡特有遗传谱系的小鼩鼱（）也呈血清阳性（1/1）。2 只 和 1 只 呈血清阴性。IFA 结果显示，鼠血清与感染汉坦病毒（HTNV）、汉城病毒（SEOV）和普马拉病毒（PUUV）的 Vero E6 细胞的抗原交叉反应较弱。这些结果表明，斯里兰卡啮齿动物中存在的汉坦病毒与 THAIV 或 TRHV 相关，而与 SEOV、HTNV 或 PUUV 无关。然而，它可能与本研究中使用的原型 THAIV 株 Thai749 在血清学上有所不同。本研究表明，属于斯里兰卡特有谱系的黑线姬鼠和小鼩鼱可能是 Girandurukotte 地区 THAIV 或 TRHV 的储存宿主。需要进一步进行多个地理研究，以确认黑线姬鼠和小鼩鼱在斯里兰卡是否为 THAIV 或 TRHV 的储存宿主。
